输血后HLA及血小板相关抗体的检测及其意义  

Detection of HLA and Platelet-Associated Antibodies after Blood Transfusion and Its Meaning

作　　者：潘秀英[1] 徐开林[1] 鹿群先[1] 万美蓉[1] 徐怀善[1] 方宪勇 兰炯采[2] 米新玉[2] 郑世荣[2] 

机构地区：[1]徐州医学院附属医院 [2]中国医学科学院输血研究所

出　　处：《中国输血杂志》1993年第2期65-67,共3页Chinese Journal of Blood Transfusion

摘　　要：应用标准的微量淋巴细胞毒试验,对反复输血的患者进行了血清中 HLA 抗体筛选及特异性鉴定。同时检测了血小板相关抗体。结果 HLA 抗体的检出率为39.14%(119/304例),其中24.37%(29/119例)的抗体具有特异性,血小板相关抗体阳性率为39.83%(47/118例)。HLA 抗体的阳性频率随输血次数及供血者人数的增加而增高,差异显著。非溶血性输血反应与 HLA 抗体相关。输注浓缩粒细胞后 HLA 抗体的阳性频率最高。本文提出了一些预防及减少输血反应的措施。A standard microlymphocytotoxicity assay was used to test the sera for HLAantibodies in the patients receiving multitransfusions and to identify their speci-ficity,and at the same time the platelet-associated antibodies were detected.As a result,the dectable rate of HLA antibodies was 39.14% (119/304),of which24.37% (29/119) had specificity;the positive rat platelet-associated antibodieswas 39.83% (47/118).The frequency of positive incidence of HLA antibodiesincreased with the number of transfusions and donors,having a significant differ-ence.The nonhemolytic reaction to transfusion was associated with HLA antibodi-es.The frequency of positive incidence of HLA antibodies was the highest whenthe patients were transfused with concentrated granulocytes.There were takensome measures to prevent and reduce the transfusion reaction.

关 键 词：输血 人白细胞抗原 输血反应 

分 类 号：R457.13[医药卫生—治疗学]