昆明小鼠正常PaO_2、PaCO_2、pH值、肺系数和PQLD_(50)的测定  

Determination of Normal PaO_2,PaCO_2,pH Value,Pulmonary Coefficient and PQLD_( 50) of Kunming Mouse

作　　者：谢仰民[1] 陈韩秋 李玫 方旭 陈穗 许鸿举 

机构地区：[1]汕头大学医学院实验动物中心,汕头515031

出　　处：《中国实验动物学杂志》2001年第4期218-220,共3页Chinese Journal of Laboratory Animal Science

基　　金：广东省科学技术厅资助 (粤科计字 [2 0 0 0 ]2 12号;项目号 2KB0 770 1T)

摘　　要：目的　研究与ARDS密切相关的几个生理指标。方法　KM小鼠动物血pH、PaO2 、PaCO2 由美国NO VA血气分析直接测定 ,PQ中毒LD50 以寇氏法计算。肺系数由湿重除以体重乘 10 0 %得出数值。结果　KM小鼠正常动脉血pH值为 7 36 0 5± 0 0 6 7,PaO2 (kPa)为 12 47± 1 34 13,PaCO2 (kPa) 5 2 6± 0 4949;肺系数 (% )为 0 6 6 6 2±0 0 82 ;百草枯 (paraquat,PQ)中毒LD50 为 93 86 6mg kg。结论　以上所测定几个数据至今尚未见报道 ,我们把测定分析结果公开发表 ,填补KM小鼠理化常数方面的空白 。Objective In order to create an experimental animal model of ARDS caused by PQ poisoning,several significant biological indexes closely related to ARDS have to be surveyed in advance. Methods The pH value,PaO\-2 and PaCO\-2 of the Kunming (KM) mouse arterial blood were determined by the U.S.\|made NOVA blood gas analyses.PQLD 50 was calculated according to Coats′ method.The pulmonary coefficient was determined as wet pulmonary weight divided by body weight maltiplied by 100%. Result The normal pH value,PaO\-2 (Kpa) and PaCO\-2(Kpa) of the KM mouse arterial blood were 7\^365±0.067,12\^47±1\^3413 and 5\^36±0\^4949 respectively.Its normal pulmonary coefficient(%) was 0\^662±0\^082.The paraquat (PQ) poisoning LD50 was 93\^866*!mg/kg.Conclusion\ It was suggested that the above result might fill the gap in the field of the KM mouse physical and chemical constants and provide valuable reference data for clinical research of ARDS (acute respiratory distress syndrome) and other diseases of the respiratory system.

关 键 词：PAO2 PACO2 肺系 正常 LD50 KM小鼠 ARDS 生理指标 昆明小鼠 中毒 

分 类 号：Q954.4[生物学—动物学] R563[医药卫生—呼吸系统]