转基因技术和冷冻胚胎技术的实验数据建立  

The Establishment of Experiment Data of Transgenic and Embryo Preservation Technique

作　　者：鞠丽梅[1] 蔡有余[1] 顾熟琴[1] 余乃绚[1] 徐凤[1] 华克非[1] 三好一郎 笠井宪雪 

机构地区：[1]中国医学科学院中国协和医科大学实验动物研究所,北京100021 [2]日本东北大学实验动物部

出　　处：《中国实验动物学杂志》2001年第4期232-235,共4页Chinese Journal of Laboratory Animal Science

基　　金：国家"九五"攻关课题 ( 96 -A2 3-0 6 -0 2 )

摘　　要：目的　建立一系列转基因技术和冷冻胚胎技术的实验室数据库。方法　a)显微注射法注射 186 4个1-细胞期受精卵 ,注射后存活的 12 83个胚胎进行胚胎移植 ,获得 2 0只阳性转基因动物。b)设计不同单位的激素剂量 (PMSGHCG)获得每个品系的小鼠平均排卵量和最佳激素剂量。c)采用常规法和玻璃化法进行胚胎冷冻保存 ,并就囊胚和桑椹胚两个不同时期进行复苏成活率的比较。结果和讨论　a)建立转基因技术和胚胎冷冻保存的实验数据库 (随着实验技术的不断提高还将不断的完善 )。胚胎显微注射存活率为 6 8 8% ;转基因阳性鼠出生率为16 16 % ;基因整合率为 1 5 6 %。b)激素诱发 5种品系小鼠的平均超排卵数为 :C57BL 6J(日本 ) 15 6 ;C57BL 6J 19 6 ;BDF1(日本 ) 2 1 4;C3H(日本 ) 14 8;KM 30。c)胚胎冷冻保存复苏 ,出生幼子存活率为 30 %～ 31%。Objective To establish the technical data of transgene and cryopreservation. Method a)Five different genes were microinjected into ova, totaling 1864 1\|cell ova injected and 1283 2\|cell ova transferred. Finally we produced 20 transgenic mice. b)We got the optimum dosages of hormone for superovulation by five mouse strains injected with PMSG and HCG. Also we got average data of embryo superovulation of different strains. c)The embryo cryopreservation was produced by the normal method and glasses method. Results and Conclusion a)The integration rate of transgenic was 16.16% and the efficiency of gene transfer was 1.56%. b)The average superovulation number of different strains was 15.6 for C 57BL/6J (Japan) strain; 19.6 for C 57BL/6J; 21.4 for BDF1 (Japan) strain; 14.8 for C3H strain; 31 for KM strain. c)The ratio of the pups born after cryopreservation of mouse embryo was 30%-31%.

关 键 词：C57BL/6 阳性 激素 复苏 小鼠 存活率 剂量 胚胎冷冻保存 冷冻胚胎 转基因技术 

分 类 号：Q78[生物学—分子生物学] S823[农业科学—畜牧学]