从牛精浆中提纯神经生长因子的生物活性检测  

THE DETERMINATION OF BIOLOGICAL EFFECT OF NERVE GROWTH FACTOR PURIFIDED FROM BOVINE SEMINAL PLASMA

作　　者：方煌[1] 吴洋管 庞清江 袁太珍 罗永湘[1] 刘海雄[2] 林娜[2] 

机构地区：[1]武汉同济医科大学同济医院骨科,430030 [2]卫生部武汉生物制品所,430000

出　　处：《中国修复重建外科杂志》1993年第1期32-34,共3页Chinese Journal of Reparative and Reconstructive Surgery

基　　金：国家自然科学基金

摘　　要：神经生长因子(Nerve growth factor,NGF)是维持交感神经和感觉神经元生存、发育,并能促进神经突起生长的一种生物活性物质。牛精浆中含量极为丰富,每10ml牛精浆可提纯约0.1mg的NGF。我们改良Gregory等报道的方法,成功地从牛精浆中提纯出低分子NGF,并采用96孔培养板作鸡胚背根神经节无血清培养,对所提纯的NGF进行活性检测,显示出较高的生物活性(2～4ng/ml),进一步证实NGF对促进神经突起的生长及神经元的生存具有重要作用。It has been proved that the bovine seminal plasma contains rich source of NGF around 0.1mg of pure NGF can be isolated from 10ml bovine seminal plasma. Modifying Gregory's method, we first successfully obtained the low molecular weight form of bovine NGF in China.We chose the dorsal root sensory ganglia (DRG) of embryonic chicken as a cultured nerve tissue, the NGF purified from seminal plasma is added to cultural plate with 96 holes. The cultural process was without plus serum and showed the high biological activity.It was found that this method has the advantages of simple technique, satisfactory result. It is an ideal method for assaying the biological effect of NGF.

关 键 词：神经生长因子 牛精浆 提纯 

分 类 号：R392－33[医药卫生—免疫学]