脂质过氧化和维生素E对培养人内皮细胞的影响  

EFFECT OF LIPID PEROXIDATION AND VITAMIN E ON HUMAN ENDOTHELIAL CELLS IN VITRO

作　　者：董玉兰[1] 陈铁镇[1] 王铁吉[1] 王跃忠[1] 张亚佳[1] 

机构地区：[1]中国医科大学实验病理学研究室

出　　处：《中国循环杂志》1993年第2期98-100,共3页Chinese Circulation Journal

摘　　要：用联胺作用于培养人内皮细胞,激发并促进了脂质过氧化。随联胺浓度增加,内皮细胞过氧化脂质浓度增加,前列环素合成减少。其形态变化主要为细胞收缩,质膜泡形成,内质网扩张,线粒体肿胀,细胞空泡化及坏死脱落。抗氧化剂维生素E能显著降低联胺引起的过氧化脂质的升高,增加前列环素的合成并明显减轻内皮细胞的形态损伤。但在高浓度联胺时,维生素E无明显保护作用。这些结果提示,脂质过氧化可能通过损伤内皮细胞而在动脉粥样硬化发生中起重要作用,维生素E由于保护内皮细胞的氧化性损伤而可能有防治动脉粥样硬化的作用。Lipid peroxidation was initiated and facilitated in cultured human umbilical vein endothelial cells by treating the cells with diamide. The concentration of lipid peroxide was increased, prostacyclin synthesis was decreased in endothelial cells with increase of diamide concentration. Significant morphologic changes were cell contraction, plasma membrane blehbing, dilatation of endoplasmie reticulum and mitochondria, vacuolization and necrosis, desquamation of cells. Antioxidant vitamin E markedly lowered the increase of lipid peroxide concentration by diamide, increased prostacyclin synthesis and obviously lightened the morphologic changes of cells. But the protective effect was not significant at high concentration of diamide. The results suggest that lipid peroxide and vitamin E might play important roles in atherogenesis and anti-atherosclerosis, respectively.

关 键 词：内皮细胞 过氧化脂质 动脉粥样硬化 

分 类 号：R543.502[医药卫生—心血管疾病]