虎杖晶4号对人血PMNs呼吸暴发和氧自由基的作用  

Effects of polydatin Ⅳ on inhibiting respiratory burst of PMNs and scavenging oxygen free radicals

作　　者：金伟军 陈树元 钱忠秀 石兴华 

机构地区：[1]第一军医大学南方医院药理室,广州510515

出　　处：《中国药理学通报》1993年第5期355-357,共3页Chinese Pharmacological Bulletin

摘　　要：应用化学发光法测定①人血PMNs受PMA刺激产生的化学发光;②黄嘌呤—黄嘌呤氧化酶体系产生O_2;③VitC—CU^(2+)—酵母多糖产主·OH;④H_2O_2的释放。观察虎杖晶4号对上述体系产生自由基的影响。结果提示.虎杖晶4号对PMN呼吸暴发的早期(1～6 min)有明显的抑制作用,呈剂量依赖性;而在12min时发光强度均高于空白对照.表明因虎杖晶4号而使呼吸暴发滞后。其对O_2.·OH.H_2O_2均有清除作用.且呈剂量依赖性.IC_(50)分别为14.6.29.6和13.0μmol·L^(-1).Chemiluminescence method was used to measure: (1) active oxygen species generation induced by respiratory burst of polymor-phonuclear leucocytes (PMNs) from human blood stimulated with phorbol myristate acetate (PMA); (2) superoxide (O2) induced by xan-thine-xanthine oxidase system; (3) hydroxyl radicals ( · OH ) generated by Vit C- Cu2+- zy-mosan; (4) the release of hydrogen peroxide (H2O2). Effects of polydatin IV on these active oxygen species were observed. The results showed early stage of respiratory burst of PMNs was inhibited,but the later stage was delayed by polydatin IV, (2), (3) adn (4) were scavenged by polydatin IV and their median inhibitory concentrations (IC50μmol · L-1) were 14.6,29.6 and 13.0 respectively. The results suggested that polydatin IV was a scavenger.

关 键 词：虎杖晶4号 多形核白细胞 氧 自由基 

分 类 号：R974[医药卫生—药品]