抗青蒿琥酯钠伯氏疟原虫的增殖动力学改变  

Changes of proliferating kinetics in Plasmodium berghei of sodium artesunate-resistance

作　　者：刘爱如[1] 赵东坡[1] 隋在云[1] 吕丽莉[1] 

机构地区：[1]山东省中医药研究所,济南250014

出　　处：《中国药理学通报》1993年第6期458-460,共3页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目

摘　　要：应用荧光显微分光光度计测定了经荧光探针DAPI示踪的伯氏疟原虫单核原虫的DNA含量。结果表明疟原虫核内的DNA复制是连续的.其含量为高度的多倍性,细胞增殖周期的G_1期不明显.R原虫DNA含量分布的离散程度明显低于N原虫.峰位较集中和明显右移。提示原虫对青蒿琥酯钠产生抗性后.DNA复制的速率和细胞的增殖活力明显降低.即原虫的增殖动力学发生了改变。In the present paper, we measured DNA content of uninuclear Plasrnodium berghei traced with DAPI by means of fluoromi-crospectrophotmeter. The results indicate that the DNA replication of parasite was continuous and it's content was high polyploidy and phase G1 of proliferating cycle was not evident. The dispersion degree of distribution of DNA content in P. R. was markedly lower and the peak site was more concentrated and obviously shifted to the right compared with P. N. It was suggested that the speed of DNA replication and proliferating vitality of parasites after producing resistance to SA markedly decreased, indicating that the changes of proliferating kinetics of P. R. happened.

关 键 词：青蒿琥酯钠 疟原虫 抗药性 抗疟药 

分 类 号：R978.61[医药卫生—药品]