HPLC测定盐酸丁卡因注射液含量  

Detrmination of tetracain hydrochloride injection by HPLC

作　　者：袁锁中[1] 

机构地区：[1]北京铁路总医院

出　　处：《中国药学杂志》1993年第11期676-678,共3页Chinese Pharmaceutical Journal

摘　　要：本文报道用ＨＰＬＣ法测定盐酸丁卡因注射液的含量，并同时测定分解产物对丁氨基苯甲酸的含量。采用ＯＤＳ柱，流动相为甲醇－０．０２７５ｍｏｌ／Ｌ磷酸二氢钠（８５：１５），以磷酸调ＰＨ为４．５，流速１ｍｌ／ｍｉｎ，以对氨基苯磺酸为内标，检测波长为３００ｎｍ。盐酸丁卡因线性范围为０．１～１．０μｇ，回收率为９８．９９％，ＲＳＤ＝１．０１％；对丁氨基苯甲酸线性范围为０．０００５－０．００５μｇ，回收率为９８．Tetracain hydrochloride (Ⅰ) injection andits degradation product p-butylamino-benzoicacid (Ⅱ) was determined by HPLC. Chro-matographic conditions included instrement,Sp-8810, column size, ODS, mobil phase,MeOH-0.0275mol/L NaH_2PO_4(85:15), pH4.5 by H_3PO_4, flow-rate, lml/min, internalstandard, sulfanilic acid; detected at 300nm.The standard curve was linear in the range e-valuted at Ⅰ0.1～1.0μg, Ⅱ 0. 0005～0.005μg. The average recovery was Ⅰ98.99%, Ⅱ98.18%; RSDⅠ 1.0%, Ⅱ 1. 81%(n=3).

关 键 词：盐酸 丁卡因 高效液相色谱 含量 

分 类 号：R927.2[医药卫生—药学]