E.coli中alKA基因突变启动子及体外转录的研究  

THE PROMOTER MUTANTS AND IN VITRO TRANSCRIPTION OF alKA GENE E.COLI

作　　者：于成国[1] 陈日新[1] 马朗[1] 李素玮 

机构地区：[1]中国医科大学基础医学部生物化学教研室 [2]佳本斯卫生学校

出　　处：《中国医科大学学报》1993年第3期165-166,共2页Journal of China Medical University

摘　　要：用人工突变的方法获得aIKA基因启动子区的突变体,以该突变的启动子DNA为模板进行体外转录试验及β-半乳糖苷酶活性测定。观察到测得的β-半乳糖苷酶活性与体外转录产生的alKA mRNA相平行。The promoter mutants of alKA gene had been gained by the method of artificial mutant.The transcription of alKA gene promoter in vitro had been done and β-galactosidas activity had been determined. The activity of The enzyme and the mRNA of alKA were parallel.

关 键 词：大肠杆菌 alKA基因 启动子 转录 

分 类 号：Q754[生物学—分子生物学]