抗CD_3单克隆抗体HIT3aⅠ.制备和特异性鉴定  

A High Affinity CD3 Monoclonal Antibody HIT3a Ⅰ. Production and Identification

作　　者：沈德诚[1] 杨希峰[1] 杨春瑛[1] 汤美华[1] 张金香[1] 白金芬[1] 孙同哲[1] 段世利[1] 刘汉芝[1] 余鸣 陈辉树[1] 陈璋[1] 

机构地区：[1]中国医学科学院血液学研究所

出　　处：《中国医学科学院学报》1993年第3期157-162,共6页Acta Academiae Medicinae Sinicae

摘　　要：利用鼠—鼠杂交技术获得1个单克隆抗体(McAb)HIT3a。经免疫学、细胞化学和生物化学分析,证实HIT3a是抗入成熟T细胞表面CD_3抗原McAb,具有亲和力高、活性稳定、能激活T细胞、在补体存在下溶解T细胞等特性,是1个理想的可用于免疫学研究和临床治疗的CD_3类McAb。A monoclonal antibody (McAb, named HIT3a) against CD3 antigen of human T lymphocytes was produced by the mouse-mouse hybridization technique and identified by immunofluorescent test, immunohistochemistry assay, comparison with standardized McAbs, and some studies of biological properties. HIT3a McAb was found to have high affinity, good stability, complement fixation activity, specific cytotoxicity against T cells,and effective mito-genic activity on normal PBMCs. So it might be a good reagent for clinical and laboratory research.

关 键 词：CD3 亲和力 制备 单克隆抗体 HIT3a 

分 类 号：R392－33[医药卫生—免疫学]