TRH、IL-1β对大鼠垂体GH_3细胞游离钙影响的体外研究  

EFFECTS OF TRH AND INTERLEUKIN-1β ON CYTOSOLIC FREE CALCIUM MOBILIZATION IN RAT GH_3 PITUITARY CULTURED IN VITRO

作　　者：王晓东[1] 

机构地区：[1]中国人民解放军总医院内分泌科,100853

出　　处：《中华内分泌代谢杂志》1993年第1期38-39,共2页Chinese Journal of Endocrinology and Metabolism

摘　　要：作者使用Fura-2作为Ca^(++)指示剂,观察了促甲状腺激素释放激素(TRH)和白细胞介素-1β(IL—1β)对体外培养的大鼠GH_3垂体细胞内Ca^(++)([Ca^(++)]i)水平的影响。结果显示10^(-7)～10^(-9)mol/L浓度TRH促进[Ca^(++)]i动员,其程度呈TRH剂量依赖性。单用0.01～10U/ml IL—1β未增加工[Ca^(++)]i水平。用0.1U/ml IL—1β与待测细胞温育1和3分钟后,加入TRH,[Ca^(++)]i释放则受明显抑制;而TRH与IL—1β同时使用,抑制作用则明显减弱。本研究证实[Ca^(++)]i对TRH在垂体细胞内的作用起重要信息传递作用,一定剂量IL—1β可能通过抑制TRH诱导的[Ca^(++)]i释放影响垂体功能。The effects of TRH and recombinant human IL-1β on cytosolic free calcium mobilization in rat GH, pituitary cells cultured in vitro was studied spectrofluorometrically using Fura-2 as Ga++ indicator. The results showed that TRH 10-7～10-9mol/L stimulated the cytosolic Ca++mobilization in a dose-dependent manner. IL-1 alone in the concentration ranging from 0.01 to 10 U/ml did not increase the intracellular Ca++ level. 1- and 3-minute pretreatment of the GH3 cells with IL-1β 0.1 U/ml significantly inhibited TRH-induced Ca++ mobilization, whereas such suppressive effects were significantly diminished when IL-1/3 and TRH were added at the same time. These findings suggest that Ca++ may signal a TRH effect in pituitary cells and IL-1β influences pituitary function by inhibiting Ca++ mobilization induced by TRH.

关 键 词：白细胞介素-1 垂体 游离钙 TRH 

分 类 号：R335.1[医药卫生—人体生理学]