HLA-DQA_1位点寡核苷酸探针检测上海地区汉族IDDM易感性  

DETECTION OF IDDM SUSCEPTIBILITY IN SHANGHAI HANS WITH HLA-DQA, OLIGONUCLEOTIDE PROBES

作　　者：孙荣[1] 马骏先 罗敏[1] 陈家伦[1] 费虹明[2] 王伟成 陈仁彪 许曼音[3] 陈风生[3] 胡远峰[4] 张洪德[4] 顾芷芳[4] 范丽安[5] 

机构地区：[1]上海第二医科大学附属瑞金医院,上海市内分泌研究所200025 [2]上海第二医科大学 [3]上海市瑞金医院 [4]上海市第一人民医院 [5]上海市免疫学研究所

出　　处：《中华内分泌代谢杂志》1993年第4期199-201,共3页Chinese Journal of Endocrinology and Metabolism

摘　　要：应用聚合酶链反应(PCR)和寡核苷酸探针点杂交技术分析了中国上海地区汉族42例胰岛素依赖型糖尿病(IDDM)患者和40例正常对照的DQA_1基因。结果发现IDDM组DQA_1*0301和DQA_1*0501等位基因频率显著高于正常对照组,而IDDM组DQA_1*0103和DQA_1*0201等位基因频率显著低于正常对照组。这些表明在中国上海地区汉族人群中,DQA_1*0301和DQA_1*0501与ID-DM易感性相关联,而DQA_1*0103和DQA_1*0201则增强对IDDM的抵抗性。对DQA_1链52位氨基酸多态性分析,提示:DQA_1链52位编码精氨酸可能与IDDM易感性有关。With polymerase chain reaction and dot-blot hybridization with allele-specific oligonucleoade probes,we analyse DQA1 gene of 42 insulin-dependent diabetes mellitus (IDDM) and 40 normal controls (the Han nationality in Shanghai). We found that the frequency of DQA1 * 0301 and DQA1 * 0501 allele in IDDM patients was greatly increased, while the frequency of DQA1 * 0103 and DQA1 * 0201 allele was significantly decreased as compared with the controls. The result indicates the contribution of the DQA1 * 0301 and DQA1 * 0501 alleles to IDDM susceptibility in Han population in Shanghai. Our results also provide confirmation of the role of DQA1 * 0103 and DQA1 * 0201 to IDDM resistance in the same population.

关 键 词：聚合酶链反应 杂交 基因 糖尿病 

分 类 号：R587.103[医药卫生—内分泌]