PHA诱导人外周血淋巴细胞产生IL2的方法学及影响因素探讨  

STUDY ON IL-2 PRODUCTION OF HUMAN PBM INDUCED BY PHA

作　　者：常金丽[1] 王喻康 臧人杰[1] 

机构地区：[1]上海市免疫学研究所基础免疫Ⅱ室

出　　处：《上海免疫学杂志》1989年第1期9-12,共4页Shanghai Journal of Immunology

摘　　要：本文借用IL-2依赖细胞CTLL测定IL-2生物活性实验,检测了人外周血单个核细胞(PBM)在PHA促分裂剂的刺激下,上清内IL-2含量,并建立了一个微量的体外诱导人PBM产生IL-2的方法。实验结果示,体外诱导人PBM产生IL-2的最佳PHA浓度为1:250稀释,培养时间以44～48小时为宜。由于采用96孔板培养,诱生时需血量小。实验结果还示,诱导IL-2产生的PHA终浓度范围窄(1:250～1:500),而淋转相对宽(1:250～1:5000),单核细胞对IL-2的诱生似具有负调节作用,表现为去单核时诱生的IL-2活性增高。粗制的IL-2上清除能维持CTLL生长外,也能维持人PBM体外培养。The production of IL-2 of human peripheral blood mononuclear cells (PBM) stimulated by PHA was studied and the method of IL-2 induction in vitro has been successfully established. IL-2 content was assayed by IL-2 dependent cell-CTLL cell line. The results have shown that the optimal conditions on IL-2 induction were as follows: the concentration of PHA was 1:250 dilution, the time of culture was 44～48 hrs. The results have also shown the concentrations of PHA at 1:250 to 1:500 dilution were effective for IL-2 induction, but for lymphocytes proliferation, the concentrations at 1:250 to 1:5000 dilution,were enough. Monocytes seem to down-regulate production of IL-2. Meanwhile, we have demonstrated that the supernatants of crude IL-2 can maintain culture of PBM in vitro besides culture of CTLL cell line.

关 键 词：白细胞介素 PHA T细胞 诱导 

分 类 号：R392.11[医药卫生—免疫学]