霜剂中氢化可的松和尼泊金乙酯的高效液相色谱分析  

High Performance Liquid Chromatographic Analysis of Hydrocortisone and Nipagin in Creams

作　　者：张胜强[1] 王军[2] 许迎春[2] 俞仁爱 

机构地区：[1]中国药科大学药物分析学研究室 [2]中国药科大学 [3]浙江镇海石化医院,315207

出　　处：《中国药科大学学报》1993年第2期86-88,共3页Journal of China Pharmaceutical University

摘　　要：建立了同时测定霜剂中氢化可的松和尼泊金乙酯的高效液相色谱分析法。样品以乙醇提取,以反相C-18为固定相,甲醇-水(3:2)为流动相,紫外254 nm检测。考察了保留值与流动相组成的关系和影响测定的因素。回收率测定的结果为:氢化可的松98.42%,尼泊金99.62%。A high-performance liquid chromatographic assay procedure was established for the simultaneous deter mination of hydrocortisone and nipagin in creams using p-nitrophenol as an internal standard. HPLC was performed using a reverse-phase C-18 column and an UV detector set at 254 nm. A mobile phase containing methanol and water (3: 2) was employed. The recoveries of hydrocortisone and nipagin were 98.24% and 99.62% respectively. At the same time, the relationship between the composition of mobile phase and retention value was examined.

关 键 词：霜剂 氢化可的松 尼泊金乙酯 HPLC 

分 类 号：R927.2[医药卫生—药学]