质粒pXZ10145核苷酸序列测定和分析  

Determination and Analysis of Nucleotide Sequence of Plasmid pXZ 10145

作　　者：沈天翔[1] 贾盘兴[1] 那淑敏[1] 门大鹏[1] 

机构地区：[1]中国科学院微生物研究所

出　　处：《生物工程学报》1993年第3期216-222,共7页Chinese Journal of Biotechnology

基　　金：国家自然科学基金和科学院重点课题资助

摘　　要：以Sanger双脱氧中止法为原理,利用美国ABI公司370A自动核酸序列分析仪,我们测定了谷氨酸棒杆菌质粒pXZ10145全长4887bp的核苷酸序列,该质粒上存在包括ApaI等18种限制酶的单一切点,其它限制酶切点的数目和位置也被定出,质粒上有8个可能的阅读框架,通过序列分析,我们确定了pXZ10145断裂生成缺失突变体pNAT65的两侧位点,在两个断裂点上发现存在'ATCTAGC'7个碱基的同向重复序列。With ABI 370A autosequencer,the total nucleotide sequence of plasmid pXZl0l45 from Corynebacteria gluctamicum 1014-6T has been determined using the dideoxy chain termination method. The plasmid contains 4887 base pairs (bps). Computer aided analysis of the sequence showed the location and number of restriction enzyme cutting sites and revealed eight open reading frames (ORPs) on the plasmid. The two sites on the plasmid pXZ10145, at which deletion occured to result in plasmid pNAT65 were confirmed. At these two sites a seven base pairs sequence 'ATCTAGC' was found.

关 键 词：谷氨酸棒杆菌 质粒 核苷酸 序列测定 

分 类 号：Q939.118[生物学—微生物学]