人精子膜血小板活性因子受体的化学特性研究  

Chemical characterization of the receptor of platelet-activating factor on human spermatozoa membrane

作　　者：王春年[1] 徐红三[1] 谢文英[1] 王一飞[1] 

机构地区：[1]上海第二医科大学组织胚胎教研室,200025

出　　处：《生殖医学杂志》1993年第4期228-230,共3页Journal of Reproductive Medicine

摘　　要：用放射标记结合试验证明人精子膜上有血小板活性因子的受体存在。血小板活性因子与人类精子膜血小板活性因子受体的结合是可逆的,并在几分钟内达到平衡。按 Woolf 模型分析,得出血小板活性因子受体的解离常数(KD)为0.95±0.028 nmol/L,血小板活性因子与精子膜受体的最大结合量(B_(max))为1526±257个位点/精子。因此,血小板活性因子对人类精子作用的部位是精子膜上的相应受体。The receptors of platelet-activating factor(PAF)on human sperm membrane are investi-gated in this report.Using redio-receptor binding assay the presence of PAF-specific binding to humansperm was confirmed.Binding of PAF to the human sperm was reversible and reached equilibrium within5 minutes.Woolf analysis of PAF-specific binding to the human sperm revealed a dissociation constant(Kd)for PAF of 0.95±0.028 nmol/L and Bmax of 1526±257/sperm.The results indicate that effec-tive action of PAF on human spermatozoa is at the site of its receptors on sperm membrane.

关 键 词：血小板激活因子 受体 精子 

分 类 号：R321.1[医药卫生—人体解剖和组织胚胎学]