乳酸脱氢酶同工酶C_4与男性不育的关系  

RELATIONSHIP BETWEEN LACTATE DEHYDROGENASE ISOZYME-C_4 AND MALE STERILITY

作　　者：何剑秋[1] 金辛良[2] 周孝瑚 余振珏[1] 姚孟树 

机构地区：[1]上海医科大学基础医学院组织胚胎学教研室 [2]上海市闸北区中心医院泌尿外科 [3]华山医院泌尿外科

出　　处：《上海医科大学学报》1993年第5期347-350,T019,共5页Journal of Fudan University(Medical Science)

基　　金：高等学校科学基金委员会博士点基金(858256/210146)

摘　　要：用自制的抗人乳酸脱氢酶同工酶C_4(LDH-C_4)单克隆抗体(McAb),以免疫荧光、BA-ELISA及聚丙烯酰胺凝胶电泳(PAGE)等方法,测定了110例正常及各种不育症患者精子及精浆中LDH-C_4的含量。结果表明:不明原因性不育症、少精症及无精症患者精液中LDH-C_4的含量明显低于正常对照组,与正常组比较P值均<0.001,有极显著性差异;免疫荧光还发现不育症组伴有各种畸形精子。LDH-C_4含量的明显降低可能与男性不育有关。Using anti-human lactate dehydrogenase isozgme-O4 (LDH-O4) monoclonal antibody(McAb)with immunofluorescen.ee and BA-ELISA assay and polyacrylamide electrophoresis, the LDH-C4 content in the sperm and seminal plasma of 110 normal men and various male sterility patients were determined. Results showed that LDH-C4 contents in the semen of sterility patients of unknown causes, and azoospermia or oligospermia patients were remarkably lower than those in the normal control group, P<0.001.Immunofluoresconce also showed the existence of abnormal sperms. Consequently, the remarkeblly low LDH-C4 content might represent one of the important causes of male sterility.

关 键 词：乳酸脱氢酶 不育症 男性 ELISA 

分 类 号：R698.203[医药卫生—泌尿科学]