用氧电极快速检测枯草杆菌Rec体系对遗传毒物的反应  

RAPID DETECTION OF GENETOXINS WITH ELECTROD Bacillus subtilis Rec-ASSAY SYSTEM

作　　者：陈文芳[1] 邓家祺[1] 陈中孚[1] 

机构地区：[1]上海复旦大学化学系,复旦大学遗传学研究所

出　　处：《癌变．畸变．突变》1994年第2期43-49,共7页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家教委博士点基金

摘　　要：用测氧仪和枯草杆菌Ｍ４５、Ｈｌ７构建的开放型的生物传感系统，以卡那霉素、丝裂霉索Ｃ和谷氨酸钠为对照，对—系列化合物，包括重铬酸钾、氯化钴、醋酸铅、柔毛霉素、氨三乙酸进行检测，实验结果表明：该生物传感系统能快速、灵敏和简便地检测遗传毒物。实验结果重复性好，在环境监测致癌和诱变物质方面有实用价值。A simplified, efficient open-type biosensor system has been constructed bycombining an oxygen electrode and Bacillus subtilis rec-assay strains to detect genotoxicityof various compounds, such as daunomycin, potassium dichromate, cobalt chloride, leadacetate, sodium nitrilotriacetate using kanamycin, mitomycin C, and sodium glutamate ascontrols. The results show that the biosensor system can be used to detect genotoxic agentswith high sensitivity and fidelity. The manipulations are very simple and reproducible re-sponses can be gained within less than one hour. So this biosensor system may provide apractical tool for the screening of environmental carcinogens and mutagens。

关 键 词：遗传毒物 检测 枯草杆菌 氧电极 

分 类 号：R991[医药卫生—毒理学]