10－羟基喜树碱对人淋巴细胞和原核细胞的诱变性比较  

THE COMPAPISON OF MUTAGENICITY WITH 10-HYDROXYCAMPTOTHECIN IN HUMANLYMPHOCYTES AND IN PROKARYOTIC CELLS

作　　者：屠曾宏[1] 吴虹燕[1] 王美瑛 

机构地区：[1]中国科学院上海药物研究所

出　　处：《癌变．畸变．突变》1994年第2期15-20,共6页Carcinogenesis,Teratogenesis & Mutagenesis

摘　　要：在人淋巴细胞和原核细胞上比较了１０－羟基喜树碱（ＨＣＴ）的诱变性。用松胞素阻断的双核淋巴细胞（ＢＮＬ）来观察微核的形成．检测了浓度为０．６２５，０．１２５，０．２５和０．５０μｇ／ｍＬ的ＨＣＴ诱发ＢＮＬ的微核率．所有剂量组含微核的ＢＮＬ均明显增加并呈现剂量关系．相反ＨＣＴ浓度达３００μｇ／皿时并不诱发ＳａｌｍｏｎｅｌｌａｔｙｐｈｉｍｕｒｉｕｍＴＡ９７．ＴＡ９８，ＴＡ１００，ＴＡ１０２的菌落回变数增加．ＨＣＴ浓度为１０．２０μｇ／ｍｌ并不明显改变小牛胸腺ＤＮＡ的圆二色谱的形。这一差别可能是由於ＨＣＴ不直接作用放ＤＮＡ而涉及ＤＮＡ拓扑酶．The comparison of mutagenecity with 10-Hydroxycamptothecin (HCT) in hu-man lymphocytes and in prokaryotic cells was performed. The cytochalasiln B(CYB)-blocked binucleated lymphocytes (BNL) assay has been explored to analyze in-duced micronuclei(MN). The incidence of MN in BNL at HCT concentration of 0.062,0.125,0.25 and 0.5μg/ ml was determined. All doses of HCT yielded significant increases inMN-containing BNL and showed a dose-dependent relation. In contrast to the resultsobtailned with human lymphocytes, HCT showed no increase in revertant frequencies inthe Salmonella typhimuriun strain TA97,TA98 TA 100 and TA 102 at concentration up to300μg / plate. The effects of HCT on circular dichroism spectra of calf thymus DNA werenot observed at concentration of HCT 10, 20μg / ml. The lack of genotoxicity of HCT inprokaryotic system could be attributed its novel mechnism of action, not direct interactionwith DNA. but involving interaction with DNA topoisomerase.

关 键 词：羟基喜树碱 淋巴细胞 原核细胞 

分 类 号：R961[医药卫生—药理学]