莫合烟对小鼠胚胎细胞遗传毒性的研究  

THE GENOTOXIC EFFECT OF MO HE TOBACCO SOMKECONDENSATE ON M0USE FETUSES

作　　者：邢绥光[1] 玛依拉[1] 买尔江[1] 

机构地区：[1]新疆医学院组织胚胎学教研室

出　　处：《癌变．畸变．突变》1994年第3期22-25,共4页Carcinogenesis,Teratogenesis & Mutagenesis

摘　　要：本文用微核试验的方法检测了新疆产莫合烟（一种组制烟草）对小鼠胚胎细胞的遗传毒作用，并与新疆卷烟厂生产的天池牌香烟加以比较。给妊娠１３─１５ｄ的瑞士小鼠腹腔注射烟雾凝聚物，共二次，间隔２４ｈ，第二次染毒后１６ｈ杀死动物，取胎鼠的肝脏及母鼠的骨髓，制片，计数多染红细胞（ＰＣＥ）的微核率。结果表明莫合烟烟雾凝聚物在中等浓度（１２０ｍｇ／ｋｇ）时胎肝细胞的微核率（７．９３±２．２３）与溶剂对照（６．１７±１．８５）相比有显著性差异。骨髓细胞的微核率（８．３１±２．２１）与对照细（５．１１±１．０５）相比，亦有显著增高。在高浓度（２４０ｍｇ／ｋｇ）时，胎肝细胞的微核率上升至１２．１３±４．４３．骨髓细胞为１０．６７±４．３３，与溶剂对照相比有极显著差异（Ｐ＜０．０００５）。且均呈剂量效应关系。而天池香烟只在高浓度时胎肝细胞微核率（９．２９±２．５６）与骨髓细胞微核率（５．５±２．０７）的增高才有显著意义。两种烟相比较，在高涨度即１．３支烟／ｋｇ时，莫合烟诱发的胎肝细胞微核率大于天池香烟（Ｐ＜０．０５）。以上结果说明莫合烟雾凝聚物对小鼠胚胎细胞有遗传毒性，其毒性在高浓度时大于天池香烟。The genotoxic effect of Mo He tobacco smoke condensate(TSC)on mousefetuses and maternal bone marrow was studied employing micronucleus test and comparedwith Tian Chi cigarette smoke condensate(CSC). Swiss mice of 13-15 days gestation wereexposed to tobacco or cigarette smoke condensate by intraperitoneal injection at a 24hinterval and sacrificed 16h 16h after the second injection. Maternal bone marrow and fetallivers from each pregnant mouse were obtained for the microncleus analyses. MO He TSC at the middle(dose ( 120 mg/kg)caused a significant increase inmieronuelei in both fetal liver cells and maternal bone marrow. A higher micronucleusfrequeney was observed in fetal liver cells (12.13±4.43) and bone marrow cells (10.67±4. 33)at the dose of 240 mg/kg. It showed a significant differ(P<0. 0005 ) whencompared with the negative control . It also Showed a dose─dependent response. Only atthe high dose a significant increase in micronuclei was observed in both fetal liver and bonemarrow treated with Tian Chi CSC.At the dose of 1.3 cigarettes/kg the micronucleusfrequency of fetal liver cells induced by Mo He TSC was greater than that induced by TianCki CSC. These results indicate that Mo He Tobacco is a mutagen in mice.

关 键 词：莫合烟 烟雾凝聚物 香烟 遗传毒理 

分 类 号：R996[医药卫生—毒理学]