视黄酸对BALB／C小鼠骨髓瘤细胞增殖及6sDNA聚合酶的抑制作用  

THE INHIBITORY EFFECT OF RETINOIC ACID ON THE PROLIFERATION AND THE ACTIVITY OF THE 6S DNA POLYMERASE FROM THE MYELOMA CELLS OF BLAB/C MICE

作　　者：阎熙丰[1] 戴丰[1] 许风芝[1] 刘际清[1] 岳晓华[1] 徐平[1] 曲淑艳[1] 刘素媛[1] 潘忠诚[1] 

机构地区：[1]沈阳军区总医院老年病研究室,空军大连医院干诊科,中国医科大学生化教研室

出　　处：《癌症》1994年第2期134-137,共4页Chinese Journal of Cancer

摘　　要：本研究以终浓度３３．３μｍｏｌ／Ｌ的视黄酸和６．７μｍｏｌ／Ｌ的阿糖胞苷与ＢＡＬＢ／Ｃ小鼠骨髓瘤细胞株培养７２小时，结果细胞增殖抑制率分别为４５．３％和６１．４％。以［３Ｈ］一ｄＴＴＰ参入法法测定ＢＡＬＢ／Ｃ小鼠骨髓瘤细胞核纯化的６ＳＤＮＡ聚合酶活性，发现体外反应系统中加入终浓度为２μｍｏｌ／Ｌ，４μｍｏｌ／Ｌ，６μｍｏｌ／Ｌ，８μｍｏｌ／Ｌ，１０μｍｏｌ／Ｌ的视黄酸，６ｓＤＮＡ聚合酶活性的抑制率分别为１２．４％，１８％，２５＊％，３０，７％和３１．６％（除第１个剂量外，其余Ｐ＜０．０５～０．００１），阳性对照终浓度为６μｍｏｌ／Ｌ的阿糖胞苷的抑制率为４９％（Ｐ＜０．００１）。并对视黄酸抑癌作用的机理进行了讨论。Mydoma cells from BALB/C mice were cultured with retinoic acid at.an end-concentration of 33.3μmol/L and with cytarabine at an end-concentration of 6.7μmol/L for 72 hours,the inhibitory rates of cell proliferation were 45.3%and 61.4%,respectively. The activity of the pure 6s DNA polymerase from myeloma cells nuclear of BALB/C mice was determined by the method of(3H)dTTP incorporation,retinoic acid at an end-concentration of 2,4,6,8 and 10μ mol/L inhibited the activity of 6s DNA polymerase in vitro with the inhibitory rates of 12.4%。18%,15.6%,30.7% nd 31.6%,respectively(P<0.05～0.001,except the dose uf 2μ mol/L),the inhibitory rate of Positive control cytarabine at an end-concentration of 6μ mol/L was 49%(P<0.001)The mechan hanism of antitumor action of retinoic acid was discussed.

关 键 词：视黄酸 骨髓瘤细胞 6s DNA 聚合酶 

分 类 号：R965.1[医药卫生—药理学]