存档肝癌、肝硬化蜡块组织中HCV－RNA的检出  

DETECTION OF HEPATITIS C VIRUS RAN IN FORMALINN- FIXED,PARAFFINEMBEDDED LIVER TISSUES FROM PATIENTS WITH HEPATOCELLULAR CARCINOMA AND LIVER CIRRHOSIS

作　　者：杨建民[1] 刘为纹[1] 晋华源[1] 

机构地区：[1]重庆第三军医大学西南医院消化内科

出　　处：《癌症》1994年第4期299-301,共3页Chinese Journal of Cancer

摘　　要：以逆转录——巢式聚合酶链反应检测５６例肝细胞癌（ＨＣＣ）和１４例肝硬变（ＬＣ）福尔马林固定、石蜡包埋肝组织中的丙型肝炎病毒（ＨＣＶ）ＲＮＡ，并与其中２７例ＨＣＣ病人的血清ＨＣＡＲＮＡ进行比较。发现在组织与血清配对的２７例ＨＣＣ中，有２２例组织与血清检出结果完全一致ＨＣＶＲＮＡ在５６例ＨＣＣ中共检出２１例（３７．５％），在１４例ＬＣ中检出４例（２８．６％）。表明这种存档组织用于ＨＣＶ感染的回顾性研究是可行的，提示ＨＣＶ感染在ＨＣＣ发生中可能起着重要作用。Hepaitis C virus(HCV)RNA in formalin-fixed,paraffinembedd-ed liver tissues from patients with hepatocel lular carcinoma(HCC,n=56)and livercirrhosis (LC,n=14) was examined by the reverse transcription and nested polymerasechain reactio.(PCR)At the same time,a comparative odbservation was taken onHCV RNA between such fixed, stored liver tissues and sera in 27 cases in the samegroup of patients with HCC. It was shown that same results were observed betweenthe tissues and sera in 22 of 27 HCC patients. HCV RNA was detectable in 21 of 56 HCC patients(37.5%)and 4 of 14 LC patients(28.6 % ).The findings suggested thatsuch fixed and stored tissues may be used fror the rectrospective study of HCV infe-ction,and HCV infection may play an important role in the develompment of HCC.

关 键 词：病毒 聚合酶链反应 肝肿瘤 肝硬变 

分 类 号：R446.9[医药卫生—诊断学]