红细胞免疫印迹法检测抗SSA抗体  

Characteristics and technical problems of RBC-immunobl otting in detection of anti-SSA antibodies

作　　者：刘学明[1] Mout.,HM 

机构地区：[1]白求恩医大三院中心实验室

出　　处：《白求恩医科大学学报》1994年第1期89-91,共3页Journal of Norman Bethune University of Medical Science

摘　　要：本文以人红细胞（ＲＢＣ）胞浆提取物为ＳＳＡ抗原来源，探讨了免疫印迹法（ＩＢＴ）检测抗ＳＳＡ抗体的可能性及特点。结果显示，在ＲＢＣ－ＩＢＴ中有两条抗ＳＳＡ（干燥综合征抗原）抗体特异性酶染色带，分子量分别为５２ＫＤ和６０ＫＤ。非特异性染色带极少或无。也未见其它几种常一自身抗体（抗ＳＭ、ＲＮＰ、ＳＳＢ等）的显色带。这说明ＲＢＣ－ＩＢＴ对抗ＳＳＡ抗体的检测有高度的专一性和特异性，值得推荐。文中还就ＲＢＣ－ＩＢＴ法中主要的实验影响因素进行了探讨。Using human red blood cells as the source of SSA antigen,,we investigated thepossibility and characteristics of immunoblotting technic in the detection of anti- SSA antibodies.The results showed that two specific enzyme-stainning bands of anti-SSA antibodies were seen in the position of 52KD and 60 KD respectively None or only less non-specific bands can be found in most sera.Futhermore,We could not see any bands of other common antibodies(such as anti-SM, RNP, SSB) in this sysyem.Obviously,the bands of anti- SSA antibodies were easily to berecognized.RBC-immunoblotting seemed to be a special and recommendable method in the detec-tion of anti-SSA antibodies.However,attention should be paid to several technical problems in this system.

关 键 词：免疫印迹 干燥综合征 抗原 

分 类 号：R392.11[医药卫生—免疫学]