固相化葡萄糖氧化酶柱的研制  

Study on Immobilized Glucose Oxidase Columns

作　　者：姚凤姬 蔡梅 黄亚玮 葛雪 慈云祥 汪勤卫 

出　　处：《北京大学学报（自然科学版）》1994年第2期169-172,共4页Acta Scientiarum Naturalium Universitatis Pekinensis

摘　　要：本文报导将葡萄糖氧化酶固定在改性硅胶上的最适宜条件及用此固相化酶测定葡萄糖的条件。同时拟定了模拟酶代替辣根过氧化物酶进行催化显色的反应条件。用所建立的分光光度法在固相化酶柱上测定了血清中葡萄糖的含量。与试剂盒测定结果对照，相关性较好，回收率为９５％～１０３％。酶柱历时两年多，重复测试５００次仍未失活。The immobilization method for glucose oxidase bound to modified silica gel wasdescribed. A column with immobilized GOD was prepared and applied for the determina-tion of glucose in Human Serum. Hydrogen peroxide produced by immobilized enzymewas colorimetrically determined using horseradish peroxidase (HRP) or mimetic peroxi-dase as a catalyst. The recovery was 95%～103%. The immobilized enzymes were suffici-ently stable for at least two years.

关 键 词：固相化酶 模拟酶 葡萄糖气化酶 

分 类 号：Q532.3[生物学—生物化学]