黄瓜黑星病抗病性离体子叶接种鉴定方法  

Studies on Method for Identification of Resistance to Scab in Cucumber

作　　者：许勇[1] 朱其杰[1] 张旭 周强[1] 

机构地区：[1]北京农业大学园艺系,北京100094

出　　处：《北京农业大学学报》1994年第1期31-34,共4页

基　　金：属"八五"国家与北京市攻关项目内容

摘　　要：报道了一种新的黄瓜黑星病抗病性鉴定方法——离体子叶接种法,并对接种鉴定条件进行了研究。该方法将刚展平的子叶剪下铺到有二层滤纸的培养皿内保湿,(?)滴管将10~4～10~5个·mL^(-1)孢子浓度的悬浮液,点滴接种到离体子叶的上表皮,每滴约0.02 mL.在15～20℃,黑暗条件下鉴定,接种后5 d 调查病情。这一方法简单、可靠、不影响幼苗继续生长。A new method for screening cucumber cultivars for resistance to scab,method of detached- cotyledon inoculation was reported.The conditions of inoculation and screening were examined.The newly-expanded cotyledons were cut and placed in petri dishes with 2 layers of fitter paper wetted with wa- ter.A 0.02 mL inoculum droplet(10~4～10~5 spores·mL^(-1))was inoculated on the upper surface of each cotyledon with a dropper.Inoculated cotyledons were incubated in darkened condition at(15～20)C tem- perature.The disease indexes were read 5 days after inoculation.This method was reliable and efficient and didn't affect the growth of young plants.

关 键 词：黄瓜 黑星病 抗病性 鉴定 

分 类 号：S436.421.1[农业科学—农业昆虫与害虫防治]