华癸根瘤菌的系统发育地位  

Phylogeny of Rhizobium huakuii Determined by Partial 16S rRNA Gene Sequencing

作　　者：李英波[1] 汪恩涛[1] 冯继东[1] 陈文新[1] 

机构地区：[1]北京农业大学生物学院,北京100094

出　　处：《北京农业大学学报》1994年第2期115-119,共5页

基　　金：本文为自然科学基金的研究内容

摘　　要：16S rRNA 序列测定是细菌分类学的基本方法之一,其序列差异是确定细菌属间及属上系统发育关系的主要标准。本文根据国际系统细菌学委员会根瘤菌分委员会的建议,采用聚合酶链反应扩增了华癸根瘤菌(Rhizobium huakuii)模式菌株103的16S rRNA 基因中约260个碱基的一个片段,并对其进行了序列测定。所得序列与其他已知根瘤菌种、属的相应片段进行了比较。结果表明,华癸根瘤菌与豌豆根瘤菌和苜蓿根瘤菌两种及与其他属的碱基差异在6.9%～14.1%(17～35个碱基),相当于根瘤菌科内已知属间的差异。结合表型特征,我们认为该种可能代表了根瘤科的一个新属。This paper reported the partial 16S rRNA sequencing of strain 103,the type culture of Rhizobium huakuii,by polymerase chain reaction(PCR).The sequence obtained from this research was compared with those of relative bacteria that had been published.The distance was calculated and a phylo- genetic tree was drawn based on the sequence data.The differences in the compared fragment were 17,16 ,22,34 and 33 bases between Rhizobium huakuii and R.meliloti,R,leguminosarum,Agrobacterium tumefaciens,Azorhizobium caulinodans and Bradyrhizobium japonicum,respectively.With the considera- tion of the unique phenon shown in numerical taxonomy and the result in this research,it was assumed that Rhizobium huakuii could represent a new genus of root nodule bacteria.

关 键 词：根瘤菌 RRNA序列 系统发育 

分 类 号：Q939.114[生物学—微生物学]