抗胃转移性腺癌细胞系单克隆抗体的制备及鉴定  

PRODUCTION AND CHARACTERIZATIPN OF FOUR MONOCLONAL ANTIBODIES TO HUMAn GASTRIC ADENOCARCINOMA CELL LINE

作　　者：李成刚[1] 林三仁[1] 杨雪玲[1] 丁晔[1] 王立新[1] 

机构地区：[1]北京医科大学第二医院消化疾病研究中心,辽宁省抚顺市第二医院内科

出　　处：《北京医科大学学报》1994年第6期447-449,共3页Journal of Peking University(Health Sciences)

摘　　要：用胃转移性腺癌细胞系ＳＧＣ７９０１免疫ＢＡＬＢ／Ｃ小鼠，取脾与ＳＰ２／０骨髓瘤细胞融合，筛选出４株抗胃癌单克隆抗体，分别命名为ＢＭＧ１，ＢＭＧ２、ＢＭＧ３和ＢＭＧ４。经间接细胞免疫荧光和ＡＢＣ免疫组化染色鉴定，这组单抗与胃癌细胞系和胃癌组织有高的反应性，其中ＢＭＧ１、ＢＭＧ３与中分化腺癌及低分比腺癌的反应性分别为８４．６％和８３．３％；与正常胃粘膜腺体不反应；与部分异型增生腺体呈阳性反应。初步认为，此组单抗在胃癌的组织学和体液诊断方面有一定应用价值。our monoclonal antibodies disignated BMGs were generated by immunizing BALB/c mice with the SGC7901,A gastric adenocarcinoma cell line. 123 cases of gastric specimen were studied by immu- nohistochemistry. BMG1 and BMG4 reacted with 77.8%and 81.3%of the well differentiated adenoca- cinoma,moderately differentiated adenocarcinoma and the reactivity of 84.6%and 66.7%,respecti- vely;83.3%of poorly differentiated adenocarcinoma and 71.4%of mucinous adenocarcinoma were stained by BMG3.The MAbs did not react with normal gastric tissues.The corresponding antigens were consi- dered as glycoproteins expressed on the cell membrane.

关 键 词：单克隆抗体 制备 胃肿瘤 胃癌细胞株 SGC7901 

分 类 号：R392－33[医药卫生—免疫学] R730.3[医药卫生—基础医学]