大鼠抗小鼠胸腺基质细胞单克隆抗体的研制与鉴定  

RESEARCH ANALYSIS MAKING AND THEIR CHARACTERIZATION OF MONOCLONAL ANTIBODIES SPECIFIC FOR MURINE THYMIC STROMAL CELL

作　　者：农高惠 陈慰峰[1] 吴江声[1] 张兰芬[1] 

机构地区：[1]北京医科大学免疫学教研室,北京医科大学组织学胚胎学教研室

出　　处：《北京医科大学学报》1994年第1期3-6,共4页Journal of Peking University(Health Sciences)

基　　金：洛氏基金会中华医学基金及国家自然科学基金

摘　　要：采用脾内免疫法，制备出两组大鼠抗小鼠胸腺基质细胞（Ｔｈｙｍｉｃｓｔｒｏｍａｌｃｅｌｌｓ，ＴＳＣ）的大－小鼠Ｂ细胞杂交瘤，已获４０个单抗分泌细胞株。小鼠胸腺切片免疫组织化学染色结果：（１）髓质ＴＳＣ反应阳性，皮质ＴＳＣ反应阴性；（２）皮、髓质ＴＳＣ反应均呈阳性且强度相似；（３）髓质ＴＳＣ反应强于皮质ＴＳＣ；（４）皮质ＴＳＣ反应强于髓质ＴＳＣ；（５）胸腺细胞的反应呈弥漫性。本实验结果不仅有利于鉴别ＴＳＣ亚群并且对研究Ｔ细胞发育有重要意义。By means of intrasplenic immunization,two series of rat anti-MTSC(mouse thymic stromal cells)hybridoma have been established and 40 lines which secret MCAb have been obtained.Supernatant from MCAb,screting cell lines have been identified by immunohistochemical staining of mouse thymic section(1) react only to medullary TSC,not to cortical TSC. (2)react with cortical and me-dullarv TSC equally.(3) stronger reaction to cortical TSC than to medullary TSC.(4) stronger reac- tion to medullary TSC than to cortical TSC.(5) react to both TSC and thymocytes Our result is not only useful to the identification of TSC subset but also important to the investigation of T cell deve-lopment

关 键 词：胸腺 基质细胞 单克隆抗体 研制 

分 类 号：R392－33[医药卫生—免疫学]