早期培养人胎胸腺基质细胞的组织化学和免疫组织化学观察  

THE HISTOCHEMICAL AND IMMUNOHISTOCHEMICAL OBSERVATION ON THYMIC STROMAL CELLS OF HUMAN FETUS IN EARLY CULTURE

作　　者：孙品伟[1] 吴江声[1] 张兰芬[1] 陈慰峰[1] 

机构地区：[1]北京医科大学组织学胚胎学教研室,北京医科大学免疫学教研室

出　　处：《北京医科大学学报》1994年第2期93-95,共3页Journal of Peking University(Health Sciences)

基　　金：国家自然科学基金和美国洛氏基金

摘　　要：１３例胸腺组织取自３．５至４个月新鲜死亡人胎，剪碎后种在培养瓶内。所用培养液为ＤＭＥ，另加５％胎牛血清、１０％新生牛血清及Ｄ－缬氨酸６５０ｍｇ／Ｌ早期培养中出现两类不同形态的细胞。上皮样细胞的ＮＥ、ＡｃＰ活性及ＰＡＳ反应较弱，ＡＴＰ酶和５－ＮＴ活性较强，角蛋白呈阳性。成纤维样细胞显示较强的ＮＥ、ＡｃＰ活性及ＰＡＳ反应，Ｖｌｍｅｎｔｉｎ呈阳性。３例培养中均含两类细胞，表明培养细胞仍为胸腺基质细胞的混合体。hymic tissues(three cases )were obtained from 3.5- to 4- month-old fresh dead human fetuses. Thetissres were minced into pieces and were seeded in glass flasks.Cell culture medium is Dulbecco s mo- dified Eagle s medium(DME) suppiemented with 5% fetal bovine serum and l0%newborn calf serum and D-valine,650 mg/liter.The polygonal epithelium-like cell and spindle fibroblast-like cells were present in early cultures . In epithelium-like cells the activities of non-specific esterase and acid phospha- tase and PAS reaction were weak,the activities of ATPase and 5’-nueleotidase were strong, and kera- tin( AC1)was expressed. Fibroblast- like cells displayed the high activity of non-specific esterase and acid phosphatase,and the strong PAS reaction,and the presence of vimentin(AV3). As two cell types were present in all of the cultures of three cases,it indicated the cultured cells were a mixture of thymic stromal cells.

关 键 词：细胞培养 胸腺基质细胞 人胎 

分 类 号：R392－33[医药卫生—免疫学]