早熟染色体凝集技术研究~60Coγ射线照射对CHL细胞染色体的损伤及修复  

THE STUDY ON CHROMOSOME DAMAGE OF CHL CELLS INDUCED 60COγIRRADIATION BY PCC TECHNIQUE

作　　者：杨新海[1] 饶用清[1] 

机构地区：[1]北京医科大学放射医学教研室

出　　处：《北京医科大学学报》1994年第3期171-173,共3页Journal of Peking University(Health Sciences)

摘　　要：用ＰＣＣ技术研究６０Ｃｏγ射线照射后ＣＨＬ细胞染色体的损伤及修复，并同常规法的检测结果进行比较。结果表明，ＰＣＣ法观察的染色体损伤主要以断片为主，对Ｇ１ＰＣＣ、Ｇ２ＰＣＣ的断片数分别拟合其量效关系均符合二次多项式。ＰＣＣ的损伤检出率较常规法高１０倍左右，表明其更加敏感，更能反映初始损伤情况。he cytogenetic effects of 60 Coγ irradiation on CHL cells were simultaneously investigated by pre- mature chromosome condensation(PCC)tchnique. The results showed that the relationship between dose and response as total chromosome aberrations and Gi or G2 PCC damage could be accurately expr-essed by quadratic equation. The yield of chromosome fragments observed by PCC was 4- 10 times higher than that of conventional chromosomeaberration analysis(CCAA).This suggests that the pCC technique is more sensitive to determine the chromosome damage than CCAA.The PCC technique par-ticularly may be useful for determining the initial damage of chromosome .

关 键 词：Coγ射线 CHL细胞 染色体损伤 

分 类 号：R818[医药卫生—放射医学]