马尾松毛虫质型多角体病毒在昆虫细胞系中的离体增殖研究  

IN VITRO REPLICATION OF DENDROLIMUS PUNCT ATUSCYTOPLASMIC POLYHEDROSIS VIRUSIN DIFFERENT INSECT CELL LINES

作　　者：沈中建[1] 乐云仙[1] 陈梅琴 苏德明 

机构地区：[1]复旦大学生命科学学院病毒学研究室

出　　处：《病毒学报》1994年第1期44-50,共7页Chinese Journal of Virology

基　　金：国家自然科学基金

摘　　要：本文报道了马尾松毛虫质型多角体病毒（简称ＤｐＣＰＶ）在５株昆虫细胞系，即棉铃虫卵巢细胞系ＳＦＥ－ＨＡ－８３１与ＳＦＥ－ＨＡ－８２１２、家蚕细胞系Ｂｍ－Ｎ、粉纹夜蛾细胞系ＴＮ－３６８和草地贪夜蛾细胞系Ｓｆ－２１中的增殖。证明ＤｐＣＰＶ可在ＳＦＥ－ＨＡ－８３１、ＳＦＥ－ＨＡ－８２１２、ＴＮ－３６８和Ｓｆ－２１４株细胞系中增殖。其中ＳＦＦ－ＨＡ－８３１细胞能同时复制几种型混合的ＤｐＣＰＶ；细胞的感染率在２０％－３５％左右，每个感染细胞约可产生５０－１００个多角体；感染细胞己传代２０次以上；离体和活体增殖的病毒的形态、感染力和核酸带谱等特征均保持稳定。his paper described the replication of Dendrolimus punctatus cytoplasmic polyhedrosis vi-rus (DpCPV)in five insect cell lines.Four of these were permissive to DpCPV replication andlarge amount of polyhedra were produced.These were the cotton bollworm Heliothis armigeracell lines,SFE-HA-8212 and SFE-HA-831;the Trichoplusia ni cell line,TN-368,and theSpodoptera frugiperda cell line,Sf-21.However,no polyhedron was observed in the silk-worm Bombyx mori cell line,Bm-N.The percentage of infected cells amounted from 20% to more than 35%in different cell lines and these were serially subcultured for more than 20subcultures.This fact showed that DpCPV was persistent in cell culture.The morphology of thevirus particles,their infectivity to the cotton bollworm ,and electrophoretic migration patterns ofthe genome segments of in vitro produced virus remained unaltered as that of in vivo pro-duced virus.

关 键 词：马尾松毛虫 质型 多角体病毒 增殖 

分 类 号：S763.853[农业科学—森林保护学]