中国河南株丁型肝炎病毒全基因组的cDNA克隆和序列分析  

MOLECULATR CLONING AND SEQUENCING OF AN ISOLATE OF HEPATITIS DELTA VIRUS FROM HENAN CHINA

作　　者：刘善虑[1] 詹美云[1] 谭文杰[1] 

机构地区：[1]中国预防医学科学院病毒学研究所

出　　处：《病毒学报》1994年第2期97-107,共11页Chinese Journal of Virology

基　　金：国家863高科技生物技术

摘　　要：从我国河南－抗丁型肝炎病毒抗原（ａｎｔｉ－ＨＤＡｇ）及丁型肝炎病毒（ＨＤｖ）ＲＮＡ双阳性的ＨＢｓＡｇ携带者血清中提取ＲＮＡ，采用人工合成的引物进行逆转录和聚合酶链反应（ＰＣＲ），获得了贯穿ＨＤＶ全基因组的６个相互重叠的ｃＤＮＡ片段。经双脱氧末端终止法进行核苷酸序列分析，得到了长度为１６７４ｂｐ的我国人河南株ＨＤＶｃＤＮＡ全序列。计算机分析表明，该株与我国台湾株（ＨＤＶＩＡ型）、美国－１株（ＨＤＶＩＢ型）、日本－１株（ＨＤＶⅡ型）和秘鲁－１株（ＨＤＶⅢ型）的核苷酸同源性分别为的９４．３％、８６．８％、７５．４％和６６．３％，氨基酸序列的同源性分别为８９．７％、８５．１％、７１．９％和６４．６％，并在核苷酸和推导的ＨＤＡｇ氨基酸序列中分别发现了５个和２个集中保守的区域。这些区域均与ＨＤＶ的某些重要功能密切相关。complete nucleotide sequence of an isolate of hepatitis delta virus ,which is 167bp in length ,is determined by cloning and sequencing six overlapping cDNA fragments ,from a HBsAg carrier positive both for anti- HDAg and HDV RNA from central China ,Henan province,using reverse transcription and Polymerase chain reaction. Comparison of this Chinese isolate with other known HDV isolates Taiwan(genotype ⅠA)、US-1(genotype ⅠB )、Japan -1(genotype Ⅱ)、and Peru-1(genotype Ⅲ)showed 94.3%、86.8%、75.4%、and 66.3%homology for nucleotide,and 89.7%、85.1%、71.9%and 64.6%homologyfor amino acid, respectively ,Five conserved regions in the nucleotide sequence and two conserved regions in the HDAg amino acid sequence were located ,which are crucial for some important functions of hepatitis delta virus .

关 键 词：丁型肝炎病毒 逆转录 CDNA 分析 

分 类 号：R373.21[医药卫生—病原生物学]