中国脊髓灰质炎病毒中Ⅱ_（17）和中Ⅲ＿2疫苗株部份基因序列的分析  

PARTIAL NUCLEOTIDE SEQUENCE OF VP1 FRAGMENT OFCHINESE POLIOVIRUS VACCINE STRAIN ZHONG Ⅱ17AND ZHONG Ⅲ2*

作　　者：张礼璧[1] 原稔 米山彻夫[1] 郑红[1] 荻原昭夫 

机构地区：[1]中国预防医学科学院病毒学研究所,日本国际协力事业Polio项目组,日本国立预防卫生研究所

出　　处：《病毒学报》1994年第3期235-239,共5页Chinese Journal of Virology

基　　金：自然科学基金

摘　　要：对中国脊髓灰质炎Ⅱ型减毒活疫苗株──中Ⅱ１７和Ⅲ型减毒活疫苗株──中Ⅲ２的ＶＰ１段部份核酸片段进行序列分析，并分别与相应的Ｓａｂｉｎ２和Ｓａｂｉｎ３株有关片段的核苷酸序列进行比较。在４１６个核苷酸序列中，中Ⅱ１７与Ｓａｂｉｎ２有９９．３％同源性，中Ⅲ２与Ｓａｂｉｎ３株完全一致。从我国服用国产活疫苗地区急性弛缓性麻痹病例中分离到的部份２型疫苗株，分别与中Ⅱ１７和Ｓａｂｉｎ２相比较，发现与中Ⅱ１７株更为近缘，海南、山东、湖南株分别只有０、１或２个核苷酸的改变。part of polio VPl nucleotide sequence of the Chinese polio vaccine strains ZhongⅡ17 and Zhong Ⅲ2 were analysed.Comparsion of the 417bp nucleotide sequence ofZhong Ⅱ17 and Sabin 2 showed,there are 3 nucleotide miss matches,the homology is 99.30%. TheZhong Ⅲ2 sequence is the same as Sabin 3 vaccine strain. Three vaccine like strains ofpoliovirus type 2 isolated in Hainan, Shandong,Hunan provinces were similarly analysed,these strains had 0,1 and 2 nucleotide miss matches respectively with Zhong Ⅱ17.

关 键 词：脊髓灰质炎 减毒活疫苗 病毒 序列 

分 类 号：R373.22[医药卫生—病原生物学]