萤火虫萤光素酶基因构建BIV－LTR启动子表达研究体系  

CONSTRUCTION OF BIV -LTR PROMOTER EXPRESSION SYSTEM WITH FIREFLY LUCIFERASE GENE

作　　者：刘国文[1] 纪永刚[1] 梁臣[1] 刘淑红[1] 陈启民[1] 耿运琪[1] 

机构地区：[1]南开大学生命科学学院,中国科学院微生物学研究所

出　　处：《病毒学报》1994年第4期377-380,共4页Chinese Journal of Virology

基　　金：国家自然科学基金资助项目

摘　　要：萤火虫萤光素酶基因构建ＢＩＶ－ＬＴＲ启动子表达研究体系刘国文，纪永刚，梁臣，刘淑红，陈启民，耿运琪（南开大学生命科学学院天津３０００７１）关键词萤光虫萤光素酶基因（ｌｕｃ），ＢＩＶ－ＬＴＲ，ＢＩＶ－ｔａｔ目前使用最广泛的报道基因是细菌的氯霉素乙酰转移...hybrid plasmid pBLTR- luc was constructed,which contains firefly luciferase gene under the control of BIV LTR promoter. Luciferase activity could be detected while EBL-1cells were transfected with pBLTR-luc. Plasmid pBIV tat could significantly transactivate theexpression of luciferase gene,which implied that LTR could work normally . Luciferase activity could obviously be detected when EBL- 1 cells were transfected with plasmid DNA as less as10ng.Compared with the LTR-CAT assay system, LTR-luc assay system is more rapid,simpler and more sensitive.

关 键 词：萤光虫 萤光素酶基因 牛免疫缺陷病毒 抗病毒药 

分 类 号：R978.7[医药卫生—药品]