水貂阿留申病毒体外中和试验的研究  

STUDIES ON IN VITRO NEUTRALIZATION OF MINK ALEUTIAN DISEASE PARVOVIRUS

作　　者：吴威[1] 聂金珍[1] 

机构地区：[1]中国农业科学院特产研究所

出　　处：《病毒学报》1994年第4期334-338,共5页Chinese Journal of Virology

基　　金：国家自然科学基金

摘　　要：首次应用乙醚处理的阿留申病毒（Ａｌｅｕｔｉａｎｄｉｓｅａｓｅｖｉｒｕｓ，ＡＤＶ）Ｇ株（ＡＤＶ－Ｇ）与阿留申病毒阳性血清进行体外中和试验获得成功。试验证明，经乙醚处理后ＡＤＶ抗原决定簇充分暴露，使ＡＤＶ能有效地被特异性抗体中和。水貂在感染ＡＤＶ１０天即可产生中和抗体，６０天中和抗体滴度高达１２８００。乙醚处理的ＡＤＶ－Ｇ可被兔抗ＡＤＶ结构蛋白标准阳性血清和纯化的抗ＡＤＶＦａｂ片段所中和，说明ＡＤＶ中和抗原决定簇主要位于结构蛋白上。比较了对流免疫电泳（ＣＩＥＰ）、免疫组织化学法（Ｉｍｍｕｎｏｈｉｓｔｏｃｈｅｍｉｔｒｙ，ＩＨＣ）和γ－球蛋白含量测定的结果，证明中和试验是诊断阿留申病最敏感的方法。同时，阐明了各检测方法间的相关性。his paper first report that ADV-G treated with ether can be neutralized by anti-ADV antibodies in vitro.The experiments indicated that after treatment with ether,ADV-G antigen epitopes were fully exposed and the virus can be effectively,rapidly neutralized by specific anti-ADV antibody.Minks produced neutralization antibodies 10 days post infection,and the antibody titer rose to 1∶128,800 60 days post infection.ADV-G treated with ether could be neutralized by rabbit anti-ADV structural protein antibodies in vitro,indicating that ADV antigenic determinants responsible for neutralization are located (on)ADV structural proteins.ADV-G treated with ether could also be neutralized by purified anti-ADV Fab fragment.Compared with CIEP,immunohistochemitry and gammaglobulin assay,the in vitro neutralization test of Aleutian disease virus is the most sensitive method.

关 键 词：水貂 阿留申病毒 中和试验 

分 类 号：S858.953[农业科学—临床兽医学] S852.65[农业科学—兽医学]