桑树植物防卫素研究：抗桑黄化型萎缩病品种育2号枝皮的分析  

STUDIES ON PHYTOALEXIN OF MULRERRY:PHYTOALEXIN ANALYSIS FROM MULBERRY BARK OF MYDD RESISTANT VARIETY──YU-2

作　　者：徐亚明[1] 顾云龙[1] 武济民[1] 陈培根[1] 蒯元璋[1] 夏志松[1] 

机构地区：[1]中国科学院上海药物研究所,中国农业科学院蚕业研究所

出　　处：《蚕业科学》1994年第2期77-79,共3页ACTA SERICOLOGICA SINICA

基　　金：国家自然科学基金

摘　　要：以Ｆｕｓａｒｉｕｍｓｏｌａｎｉｆ．ｓｐ．ｍｏｒｉ菌接种诱导的育２号桑枝皮经丙酮提取，抗菌活性追踪溶剂萃取得乙酸乙酯提取物活性部位，进一步反复柱层析和制备薄层层析得到Ｍ１、Ｍ２、Ｍ３和Ｍ４４个植物防卫素成分。其中Ｍ２、Ｍ３；和Ｍ４经波谱解析和文献对照，证明为ＭｏｒａｃｉｎＣ，Ｇ和Ｎ。The mulberry shoots of Yu-2 which were inoculated with Fusarium sclani f.sp.mori ,were extracted with acetone. The extract was then fractionated through partitionbetween organic solvents and water. Ethyl acetate extract,the active fraction,was fur-ther purified through column and preparative thin layer chromatography, and four phy-toalexins such as M1,M2,M3and M4 were obtained. Among them,M2-4 were identifiedas Moracin C,G and N by spectral elucidation and comparison with reported data in Iit-eratures.

关 键 词：桑树 黄化萎缩病 植物防卫素 

分 类 号：S888.712[农业科学—特种经济动物饲养]