鸡胚与胎鼠的成纤维细胞条件培养液促进成肌细胞增殖和融合的比较  

COMPARISON OF PROMOTING THE PROLIFERATION AND FUSION OF MYOBLAST BETWEEN CONDITIONED MEDIA FROM FIBROBLASTS OF CHICK EMBRYO AND FETAL MOUSE

作　　者：顾锦法[1] 颜贻谦[1] 周振华[1] 

机构地区：[1]中国科学院上海生理研究所

出　　处：《生理学报》1989年第2期191-195,共5页Acta Physiologica Sinica

摘　　要：用培养过鸡胚(来亨鸡)或胎鼠(ICR小鼠)肌组织的成纤维细胞的条件培养液,定量地研究它们对胎鼠或鸡胚的成肌细胞的增殖和融合的影响。所得结果如下:(1) 胎鼠的成纤维细胞条件培养液促进胎鼠或鸡胚成肌细胞增殖,分别为对照组的2.65倍,(P<0.001)或2.35倍,(P<0.01);(2) 鸡胚的成纤维细胞条件培养液促进鸡胚或胎鼠的成肌细胞增殖,分别为对照组的2.66倍,(P<0.01)或2.17倍,(P<0.01);(3) 胎鼠的成纤维细胞条件培养液增加胎鼠或鸡胚的成肌细胞的融合率,分别为对照组的1.9倍或2.6倍;鸡胚的成纤维细胞条件培养液只增加鸡胚成肌细胞的融合率,为对照组的2.1倍,但对胎鼠成肌细胞的融合无明显的影响。 实验结果提示:成纤维细胞条件培养液促进成肌细胞的增殖,两种动物间无明显的差异,但在融合上却有一定的种属特异性。Conditioned media were prepared by using the collected media which had cultured fibroblasts from muscle tissue of fetal mouse (ICR) or chick embryo (Leghorn chicken). The effects of these media on the proliferation and fusion in mouse o(?) chick myoblast were studied quantitatively.The results were as follows: (1) the conditioned medium from fibroblasts of fetal mouse promoted the proliferation of mouse or chick embryonic myoblasts by 2.65 times (P<0.001) or 2.35 times (P<0.01) as compared with control group(?) respectively. (2) The conditioned medium from fibroblasts of embryonic chicken promoted the proliferation of chick or mouse embryonic myoblasts by 2.66 times (P<0.01) or 2.17 times (P<0.01) respectively. (3) The conditioned medium from fibroblasts of fetal mouse enhanced the fused rate of mouse or chick myoblasts by 1.9 or 2.6 times respectively. The conditioned medium from fibroblasts of embryonic chick enhanced the rate of chick myoblast fusion by 2.1 times, but the effect on the mouse myoblast fusion was not remarkable.The results suggest that the effect of fibroblast conditioned media on myoblast proliferation is similar in the two species, but the effect on the fusion of myoblasts is somewhat species-specific.

关 键 词：成纤维细胞 成肌细胞 条件培养液 

分 类 号：Q954.66[生物学—动物学]