锌诱导的蛋白合成增加可抵抗链佐霉素所致的胰岛细胞损伤  

THE INCREASE OF PROTEIN SYNTHESIS BY ZINC CAN RESIST THE DAMAGE INDUCED BY STREPTOZOTOCIN ON ISLET CELLS

作　　者：屠亚红[1] 朱文玉[1] 李承红 

机构地区：[1]北京医科大学基础医学研究所消化生殖生理研究室

出　　处：《生理学报》1989年第4期375-380,共6页Acta Physiologica Sinica

摘　　要：本工作在离体细胞水平观察ZnGl_2对链佐霉素(STZ)诱发的胰岛β细胞损伤的保护作用,并分析其可能的作用机制。结果如下:向培养的胰岛细胞中加入生理盐水和STZ(3mmol/L),孵育12h后,活细胞数由实验前的70万个/ml降至43.93±1.16万个/ml;将ZnCl_2(0.25、0.5、1.0mmol/L)和相同剂量的STZ一同加入细胞,可不同程度地缓解STZ对胰岛的破坏作用,在含不同浓度ZnCl_2的培养液中活细胞数分别恢复至47.39±0.88,58.06±2.29,67.72±1.48万个/ml,与STZ破坏组相比,分别具有显著差异,并呈量效关系。在给予ZnCl_2(1.0mmol/L)的同时,向细胞中加入蛋白合成抑制剂亚胺环已酮(100μg/ml),可翻转ZnCl_2的作用,活细胞数由63.17±2.15万个/ml,又重新减至45.77±0.76万个/ml。单独加亚胺环己酮对活细胞数目无明显影响。用~3H-亮氨酸掺入实验观察ZnCl_2对胰岛细胞蛋白合成的影响发现,单独给予ZnCl_2(1.0mmol/L)仅使蛋白合成轻微增加,与盐水对照组无显著差异;在给ZnCl_2的同时加入STZ,则蛋白合成明显增多,保护组与STZ破坏组比较,差异显著。上述结果表明,增加细胞内蛋白合成,以加强细胞自身对外来损伤的修复力,可能是ZnCl_2保护胰岛细胞的机制之一。The beneficial effect of ZnCl_2 on islet cells injuried by streptozotocin was observed directly on the cultured cells and its possible mechanism was analysed. The results were as follows: (1) The number of viable cells in culture was reduced from 70×10~5/ml to 43.93±1.16×105/ml 12 hours after adding normal saline plus STZ (3.0mmol / L), and this reduction could be alleviated by adding ZnCl_2 (0.25, 0.5, 1.0mmol / L) in varying amounts and a dose-response relationship was found. The number of viable cells was returned to 47.39±0.88×10~5/ml, 58.06±2.29×10~5/ml, and 67.72±1.48×10~5 /ml respectively in the culture with ZnCl_2 of different levels. (2) The effect of ZnCl_2 was reversed when cycloheximide(100μg/ml), a protein synthesis inhibitor, was added into the cultural medium, and the number of via(?)c cells was again decreased from 63.17±2.15×10~5 / ml to 45.77±0.76×10~5 ml. No obvious effect was observed when cycloheximide was given alone. (3) The experiment of ~3H-leucine incorporation into islet cells showed that protein synthesis was increased slightly and insignificantly after adding ZnCl_2 (1.0mmol / L). However, there was a significant increase of protein synthesis in cells when ZnCl_2 (1.0 mmol/ L) was added with STZ (3.0mmol / L).The results suggest that ZnCl_2 has a protective effect on the damaged islet cells induced by STZ. The increase of protein synthesis may be one of the mechanisms involved in the action of ZnCl_2, which strengthens the repairing ability of islet cells after injury.

关 键 词：胰岛细胞 锌 链佐霉素 

分 类 号：R333.3[医药卫生—人体生理学]