下丘脑八肽胆囊收缩素对大鼠胃窦运动的作用  

EFFECTS OF CCK-8 OF HYPOTHALAMUS ON ANTRAL MOTILITY IN RATS

作　　者：宋剑雄[1] 周吕[1] 柳力公[1] 徐维[2] 陈正秋[2] 徐志卿[2] 

机构地区：[1]中国医学科学院基础医学研究所生理研究室 [2]中国中医研究院针灸经络研究所

出　　处：《生理学报》1989年第6期567-574,共8页Acta Physiologica Sinica

基　　金：国家自然科学基金

摘　　要：下丘脑外侧区(LH)和腹内侧区(VMH)微量注射10ng八肽胆囊收缩素(CCK-8)明显抑制清醒大鼠胃窦运动,这一作用可被切断隔下迷走神经所减弱,被阿托品或酚妥拉明静脉灌流所阻断,表明迷走和交感神经都介导CCK-8作用。同时,在LH注射CCK-8后,迷走背核神经元自发放电活动明显减弱。LH微量注射抗CCK-8血清则明显刺激大鼠胃运动,表明在基础状态下,内源性CCK-8对胃窦运动有持续性抑制作用。用免疫组化PAP法显示出在LH和VMH都有CCK-8免疫反应性神经元存在。Cholecystokinin-octapeptide(CCK-8)of 10ng were injected into lateralhypothalamus(LH)and ventromedial hypothalamus(VMH)in conscious rats,bothof which brought about inhibition of antral motility recorded by a strain gauge,theactions were partly antagonised by bilateral vagotomy,but blocked by intravenousinfusion of atropine or regitine.It is suggested that both vagus and sympatheticnerves be involved in mediation of function of central CCK-8.In addition,theunit spontaneous discharges of neurons in vagal neuclei were attenuated while CCK-8 injected into LH.On the contrary,the antral motility was stimulated markedlyby injection CCK-8-antiserum into LH,indicating that the endogenous CCK-8in this area exercises a continuous restraint on antral motility in the basal state.CCK-8 cells both in LH and VMH were visualized by PAP method.It may bereasonable to refer this as the histological basis of the identical function on antralmotility exercised by CCK-8 both in LH and VMH.

关 键 词：下丘脑 胆囊收缩素 胃窦运动 

分 类 号：Q579.23[生物学—生物化学]