双氧水诱导淋巴母细胞株产生EB病毒相关抗原的研究  

EBV─ASSOCIATED ANTIGEN PKODUCTION OF LYMPHOBLAST CELL LINES INDUCED BY H2O2

作　　者：尚大庄[1] 董燕[1] 李佩杰[1] 李燕[1] 谷淑燕[1] 王新江[1] 陆虹[1] 

机构地区：[1]承德医学院,中国预防医学科学院病毒所

出　　处：《承德医学院学报》1994年第3期169-172,共4页Journal of Chengde Medical University

摘　　要：研究了双氧水（Ｈ２Ｏ２）诱导淋巴母细胞株产生ＥＢ病毒相关抗原的作用条件，并观察了Ｈ２Ｏ２与丁酸钠和巴豆油联合作用于上述细胞后对ＥＢ病毒相关抗原的协同激活作用。结果表明，０．２ｍＭ的Ｈ２Ｏ２室温下处理Ｂ９５─８细胞１０分钟，培养５天后，其ＥＢ病毒壳抗原（ＥＢＶ─ＶＣＡ）阳性细胞率分别由４％和１％增至３６％，结果与丁酸钠和巴豆油单独激活时无显著差异。证明Ｈ２Ｏ２可以代替具有致突或促癌的丁酸钠和巴豆油用于激活Ｂ９５─８和Ｐ３ＨＲ─１细胞，但对Ｐ３ＨＲ─１ＥＢ─ＶＣＡ和Ｒａｊｉ细胞ＥＢＶ早期抗原（ＥＢＶ─ＥＡ）的激活不够理想。Ｈ２Ｏ２、丁酸钠和巴豆油联合作用于上述三种细胞可以获得最好的激活效果，Ｂ９５─８和Ｐ３ＨＲ─１的阳性细胞率分别可达８０％和６４％，Ｒａｊｉ细胞的ＥＢＶ─ＥＡ阳性率可达１４％。The optimal conditions for the EBV─associated antigen production of Iymphoblast cell lines induced by H2O2 and the synergetic effect of the concomitant use of H2O2,TPA and corton oil on expression of EBV─associated antigen gene of the Iymphoblasts were studied.The results showed that treatment of B95─8cells with H2O2 of0.2mmol/L at room temperature for 10 minutes and culture for 5 days could increase theEBV─VCA positivity rate from 4%to 36%.There was no significant difference betweenactivating B95─8 by H2O2 and activating it by TPA or by corton oil alone,and theferoreH2O2 can replace carcinogenic activation reagents,TPA and corton oil,for the acticationof B95─8 cells;but its effect on P3HR─1and Raji cells are not as good as that on B95─8cells.At the same conditions,however,the highest activation results can be achieved ifthe above three activation reagents are used simultaneously for activating each of thethree lymphoblast cell lines,with a EBV─VCA positivity rate of 80%for B95─8and 64%for P3HR─1,and with a EBV─EA positivity rate of 14%for Raji cells.

关 键 词：庖疹病毒 双氧水疱疹病毒 相关抗原 淋巴细胞 

分 类 号：R373.9[医药卫生—病原生物学]