化学合成的人α-心房肽基因在SUC2启动子-信号顺序控制下的表达  

EXPRESSION OF CHEMICAL SYNTHESIZED α-hANP GENE DIRECTED BY SUC2 PROMOTER-SIGNAL SEQUENCE

作　　者：秦宁 李育阳[1] 杨迪[1] 王启松[1] 

机构地区：[1]复旦大学遗传学研究所

出　　处：《生物工程学报》1989年第1期24-29,共6页Chinese Journal of Biotechnology

摘　　要：本文报道将化学合成的人α-心房肽(α-hANP)基因与酵母分泌型表达载体YFD6A21重组,使α-hANP基因在蔗糖酶基因(SUC2)启动子-信号顺序指导下,在酵母菌中合成和分泌有活性的α-hANP。放射免疫分析表明:α-hANP基因只有在葡萄糖去阻遏条件下才能得到表达,而且其表达量与细胞浓度成正比。此外,95%以上表达产物被分泌到培养液中。The Chemical synthesized α-hANP gene has been inserted into yeast expression vector YFD6△21. The expression of α-hANP gene was directed by SUC2 promcter-signal sequence. The results of radioimmunoassay showed that the yeast cells containing chimeric vector can synthesize α-hANP only at glucose derepression condition, and the expression level was related to the cell concentration. In addition, more than 95% of expressed α-hANP was secreted into the medium.

关 键 词：心房肽 SUC2启动子 基因表达 

分 类 号：Q78[生物学—分子生物学]