高胆固醇高脂肪膳食对家兔β-VLDL组成及其与巨噬细胞相互作用的影响的初步研究  

The Effect of High Cholesterol, High Fat Diet on Rabbit Plasma β-VLDL and Its Interaction With Macrophage

作　　者：冯宗忱[1] 王式平[1] 冯友梅[1] 吴万生[1] 王淳本[1] 江卫国[1] 戴五星[2] 梁驹卿[2] 

机构地区：[1]同济医科大学生化教研室 [2]同济医科大学实验医学研究中心

出　　处：《生物化学杂志》1989年第1期66-70,共5页

基　　金：自然科学基金

摘　　要：给家兔喂以1％胆固醇及10％菜油(A组)或猪油(B组)50多天后A组血胆固醇水平(824.2±265.1mg/dl)明显低于B组(1666±693.8mg/dl);A组甘油三酯水平(51.9±19.1mg/dl)亦低于B组(104±40.2mg/dl)。二组家兔的β—VLDL的脂类组成无差别,但A组β—VLDL的apoE高于B组,分别为45.2％及37.5％。高分子量apoB(apoB_h)为33.6％,低于B组β-VLDL(47.3％)。A组β-VLDL促进小鼠腹腔巨噬细胞胆固醇堆积的程度大于B组,可能与apoE含量高有关。我们认为多不饱和脂酸减轻动脉粥样硬化(As)的作用不在于改变脂蛋白构成后阻碍泡沫细胞的形成而是促进β—VLDL从体内清除。Rabbits were fed on 1% cholesterol and 10% rape seed oil (group A) or lard (group B). Plasma cholesterol and triglyceride level of group A was strikingly lower than that of group B. No difference was found between the lipid composition of p-VLDLs from both groups, however, apo E content of β-VLDL from group A was higher and apo B (higher molecular weight) was lower than that of group B. β-VLDL of group A caused greater accumlation of cholesterol in mouse peritoneal macrophage than β-VLDL of group B. We suggest that the good effect of polyunsaturated fatty acid on atherosclerosis is not resulted from decreasing accumulation of cholesterol in macrophage but from enhancing elimination of P-VLDL by the liver.

关 键 词：多不饱和脂肪 β-VLDL 巨噬细胞 

分 类 号：Q592.1[生物学—生物化学]