致倦库蚊有机磷抗性相关扩增酯酶B基因的多样性  

Diversity Amplified Esterase B Genes Responsible for Organophosphate Resitance in Culex quinquefasciatus form China

作　　者：徐建农[1] 瞿逢伊[1] 刘维德[1] 

机构地区：[1]第二军医大学基础医学部寄生虫学教研室,中国科学院上海昆虫研究所

出　　处：《第二军医大学学报》1994年第2期101-105,共5页Academic Journal of Second Military Medical University

摘　　要：本实验从佛山和成都两地的致倦库蚊群体中筛选出３个酯酶类型不同的有机磷（ＯＰ）抗性品系，ＦＳ－１、ＦＳ－２和ＣＤ－１品系。各品系的酯酶基因Ｂ扩增水平、酯酶活性和ＯＰ抗性水平三者一致。各品系酯酶Ｂ基因限制性酶切片段比较分析表明，我国的ＯＰ抗性库蚊群体中不仅有世界性分布的酯酶Ｂ１和Ｂ２，而且在佛山和成都地区各有一个新的独立扩增的酯酶Ｂ，分别命名为Ｂ６和Ｂ７。Three organophosphate resistant strains(FS-1, FS-2 and CD-1) were selected from collections of Culex quinquefasciatus from Foshan and Chengdu, China. Different esterase patterns were observed in the three strains. The numbers of esterase B gene copies were approximately 32-fold in FS-1, 8～16-fold in FS-2 and 2～4-fold in CD-1. The amplification levels of esterase B genes were correlated with esterase activities and OP resistances among the three strains.The restriction pattern comparison of the amplified region containing the esterase B gene revealed large differences among the three strains. The esterase B1 was found in FS-1 and two esterases, B2 and a new B haplotype (named B6), were detected in FS-2. Another new haplotype(named B7)was observed in CD-1. Esterase B6 and B7 had identical electrophoretic mobility, but had different restriction patterns within the regions flanking the esterase B gene, indicating that two distinct haplotypes were amplified independently in Culex populations in Foshan and Chengdu,China.

关 键 词：蚊 致倦库蚊 杀虫剂 基因扩增 

分 类 号：R979.8[医药卫生—药品]