γ－干扰素对大鼠体内约氏疟原虫子孢子入侵及红外期发育的影响  

Effect of INF-γ on the sporozoite invasion and EEF development of P.yoelii in rats

作　　者：秦晓红[1] 王兴相[1] 况明书[1] 

机构地区：[1]第三军医大学基础部寄生虫学教研室

出　　处：《第三军医大学学报》1994年第1期13-15,共3页Journal of Third Military Medical University

基　　金：国家自然科学基金

摘　　要：用葡萄球菌肠毒素Ａ刺激体外培养的大鼠脾细胞所获得的γ－干扰素（ＩＦＮ－γ），于接种约氏疟原虫子孢子前５ｈ静注于Ｗｉｓｔａｒ大鼠体内，子孢子接种４２ｈ后肝内红外期（ＥＥＦ）数量显著减少，其子孢子发育率仅为对照组的７％～２０％，但ＥＥＦ的大小及成熟度均未受影响。大鼠接种子孢子后２ｈ给ＩＦＮ－γ，则红外期的密度、子孢子发育率及红外期大小，成熟度均与对照组无明显差异。将子孢子与ＩＦＮ－γ共孵４０ｍｉｎ，然后超速离心洗脱ＩＦＮ－γ后再接种于大鼠，则红外期的密度、大小、成熟度也均类似于对照组。结果提示：ＩＦＮ－γ的抗疟作用并非是针对子孢子或红外期本身，而是通过修饰枯否氏细胞活性，减少入侵肝细胞的子孢子数来表达的。Gamma-interferon(IFN-γ)was obtained through the culture of the splenocytes of Wistar rats and the culture was stimulated with the enterotoxin A of staphylococcus. Rats were injected with INF-γ 5 hours before sporozoite inoculation. It was found that 42 hours after inoculation,the density of exoerythrocytic forms(EEF)was markedly decreased and the viability of the sporozoites was noly 7%～20% of that of the control.However,the size and maturation rate of EEF were not affected.When IFN-γ was given 2 hours after sporozoite inoculation, the sporozoite viability,the size and maturation of EEF were all normal as compared with those of the control. When sporozoites were preincubated with IFN-γ for 40 minutes and then injected into rats,the density,size and maturation rate of EEF were not affected. The findings suggest that the anti-malarial effects of INF-γ are not directly act on the sporozoits or EEF but prevent the invasion of sporozoites through the modification of the activities of Kuppffer cells.

关 键 词：疟原虫 红外期 干扰素 大鼠 发育 

分 类 号：R382.31[医药卫生—医学寄生虫学]