甲型肝炎患者外周血T淋巴细胞亚群变化的检测  

Changes of peripheral blood T lymphocyte subsets in patients with hepatitis A and its significance

作　　者：郝春秋 阎荣[1] 陈龙邦 周永兴 陈远鑫 

机构地区：[1]西京医院传染病科,西京医院临床免疫科,西京医院唐都医院传染病科

出　　处：《第四军医大学学报》1994年第1期46-48,共3页Journal of the Fourth Military Medical University

摘　　要：作者应用ＣＤ系列单克隆抗体、碱性磷酸酶抗碱性磷酸酶双桥酶法（ＡＰＡＡＰ技术）对甲型肝炎患者５２例外周血Ｔ淋巴细胞亚群的变化进行了检测及分析。发现患者急性期ＣＤ４＋细胞百分率（３０．７±３．４％）较正常对照（３７．７±２．５％）明显降低（Ｐ＜０．０１），ＣＤ８＋率则显著增高（３５．８±３．９％对２７．７±２．４％，Ｐ＜０．０１），导致ＣＤ４＋／ＣＤ８＋比值（０．８７±０．２对１．３±０．１）明显下降（Ｐ＜０．０１）且倒置。恢复期则以上变化基本恢复正常。对其中１１例典型患者进行了为期１０周的动态观察，发现丙氨酸氨基转移酶（ＡＬＴ与ＣＤ８＋细胞百分率之间存在着非常显著的正相关（ｒ＝０．９６，Ｐ＜０．０１），而与ＣＤ４＋率及Ｃ０４＋／ＣＤ８＋之间均存在显著的负相关（ｒ＝－０．９６，Ｐ＜０．０１；ｒ＝－０．９５，Ｐ＜０．０１）。以上结果在一定程度上说明了细胞免疫可能参与了甲型肝炎的发病机理。Changes of peripheral blood T lymphocyte subsets in 52 patients with hepatitis A were observed byusing CD series McAb and alkaline phosphatase anti-alkaline phosphatase immunohistochemical technique. The results showed that in the acute phase of hepatitis A,CD4 positive rate(30.7±3.4%vs37.7±2.5%)decreased obviously(P<0.01),while CD8 positive rate(35.8±3.9%vs27.7±2.4%,P<0.01)increasedobviously,and that caused CD4+/CD8+ ratio(0. 87±0.2 vs 1.3± 0.1, P<0.01) decreased obviously andeven was inverted; in convalescence,CD4+,CD8+ and CD4+/C08+ return to normal.We found ALT andCD8+ had strong positive correlation;ALT and CD4+, ALT and CD4+/CD8+ had strong negative correlationrespectively through persistent observation on 11 typical patients with hepatitis A within 1～10 weeks afteronset.In general,the results of our experiment indicate,to a certain extent,cellular immunity may be one ofthe pathologic causes of hepatitis A.

关 键 词：甲型肝炎 T细胞亚群 双桥酶法 

分 类 号：R512.61[医药卫生—内科学]