大鼠体内视网膜神经节细胞诱向因子(RGNTF)及其受体的免疫组织化学定位  

IMMUNOHISTOCHEMICAL LOCALIZATION OF RETINAL GANGLION NEURONOTROPHIC FACTOR (RGNTF)AND ITS RECEPTOR IN RAT

作　　者：周明华[1] 黄威权[1] 

机构地区：[1]香港大学医学院解剖学系

出　　处：《动物学报》1994年第1期58-62,共5页ACTA ZOOLOGICA SINICA

摘　　要：本文用ＲＧＮＴＦ单克隆抗体及抗独特型单克隆抗体的免疫组织化学反应，对ＲＧＮＴＦ及其受体在 大鼠体内的分布进行了研究．结果显示，大鼠的肾脏、肾上腺、下颌下腺、胃底腺，以及睾丸的生精细胞对 ＲＧＮＴＩＦ均呈现强阳性免疫反应，并对ＲＧＮＴＦ抗独特型单克隆抗体也呈现阳性免疫反应，表明 ＲＧＮＴＦ及其受体有较广泛的分布，这种情况与神经生长因子（ＮＧＦ）及睫状节神经诱向（营养）因子 （ＣＮＴＦ）相类似．但是，ＲＧＮＴＦ及其受体的分布特点和ＮＧＦ、ＣＮＴＦ的分布是不完全相同的，提示作者 分离的ＲＧＮＴＦ与ＮＧＦ和ＣＮＴＦ不是同源物。这样肾上腺皮质、下颌下腺的浆液腺泡及导管上皮细胞、 胃底腺上皮细胞和生精细胞不仅能够产生ＲＧＮＴＦ，也能合成ＲＧＮＴＦ受体。因此，它们对ＲＧＮＴＦ可能 有自分泌的功能，ＲＧＮＴＦ对这些细胞可能有自身调节的效应。The distribution of immunohistochemical reactivities of RGNTF and its receptor in rat were investigated by using anti-RGNTF monoclonal antibody and RGNTF anti-idiotypic monoclonal antibody. The results showed that the kidney, suprarenal gland, submandibular gland,gastric fundus and testes were stained strongly in positive immunoreaction display by the anti-RGNTF antibody and the RGNTF anti-idiotypic antibody respectively. These suggested that the RGNTF and its receptor distributed widely as the NGF and CNTF did in rat, but their specific distribution and locations were different from those Of NGF and CNTF. These results indicated that RGNTF is not homology with NGF or CNTF. Since the cells in the suprarenal cortex,the serous acinar cells and the ductile epithelium in the submandibular gland,the epithelium of gastric fundus,and the Spermatogenic cells in the testes not only produced RGNTF but also synthesized its receptor. Therefore,conclusion may be drawn that all these cells may be the RGNTF-autocrine and RGNTF-autoregulated cells.

关 键 词：大鼠 视网膜神经节 细胞诱向因子 

分 类 号：Q959.837[生物学—动物学]