荧光标记十八胺LB膜的光谱研究  

SPECTRAL STUDY ON LB FILMS OF FLUORESCENT PROBE LABELED OCTADECYLAMINE

作　　者：王文韵[1] 王新平[1] 于华[1] 何家俊[1] 徐荃[1] 侯士法 关荐伊 

机构地区：[1]中国科学院长春应用化学研究所,长春130022 [2]承德石油高等技术专科学校,承德067000

出　　处：《发光快报》1994年第1期50-55,共6页

摘　　要：正十八胺以氯化丹酰和8-苯胺基-1-萘磺酸衍生化,单独或与硬脂酸混合后拉制成LB膜,并以稳态和时间分辨荧光研究了膜中分子的微环境和微结构等光物理性质.In the present paper, the LB films of dansyl chloride (DNS) and 8-anilino-l-naph-thalene sulfonic acid CANS) derivatized octadecyl-amine(AM) were deposited on solid substrates and their photophysical behavior was studied by the steady-state and time-resolved fluorescence measurements.The surface pressure/molecular area curves for DNS-AM and ANS-AM in presence or absence of atearic acid (SA) show that solid-condensed films have formed. The collapse pressure for DNS-AM and (DNS-AM)/SA(1:9) is 30 and 58mN/m respectively; while that for ANS-AM and (ANS-AM)/SA(1:5) is 35 and 58 mN/m respectively. The A isotherms for DNS-AM and (DNS-AM)/SA are unusual with a platean. The isothermn of ANS-AM and (ANS-AM)/SA have a classical shape.No significant difference was found between solution spectra in the absence or presence of stearic acid for either DNS or ANS labeled octadecylamine. It means that the diluent stearic acid has no substantial perturbation to the spectral properties of the fluorescent derivatives. The emission bands of films have a small blue shift (25 and 13 nm for DNS and ANS systems respectively) with respect to corresponding solution bands. This blue shift likely implies that the fluorescent octadecylamine experiences more hy-drophobic microenvironment in ordered LB films than in the random state in solution.The fluorescence lifetimes for DNS-AM and (DNS-AM)/SA in deposited films and in spreading solutions are identical within the experimental error range, being 13.5 1. Ons. ANS-AM and (ANS-AM)/SA also have similar lifetime values in film and in solution (ca. 8 ns). This implies that the molecules deposited on the substrate did not change their microstructure(conformation,aggregation state and etc. ). A small tendency of lengthening of fluorescence lifetimes of molecules in film as compared to that in solution,is consistent with the small blue shift of emission bands,indicating the more hydrophobic microenvironment of film molecules.The results show that the derivatization with fluorescent probes may render t

关 键 词：正十八胺 LB膜 光谱 荧光标记 

分 类 号：O484.41[理学—固体物理]