麻疹病毒抗体的酶免疫电化学分析法研究  

Electrochemical Enzyme Immunoassay for Measles Virus(MV)

作　　者：刘娟[1] 胡荫华[1] 宋俊峰[1] 程明洁 

机构地区：[1]西安市环境保护研究所,西北大学化学系,陕西省卫生防疫站

出　　处：《分析测试学报》1994年第1期66-70,共5页Journal of Instrumental Analysis

摘　　要：建立了测定麻疹病毒抗体的酶免疫电化学分析法。其原理是在异相酶联免疫之后用极谱法检测酶促产物。本法采用小型三电极系统“ＤＭＥ－Ｐｔ－Ａｇ／ＡｇＣｌ”直接与商品酶联板配套使用，简化了操作步骤。对测定麻疹抗体的合适条件以及灵敏度，特异性等作了考察。结果表明，本法特异性强，灵敏度高，检测范围宽，不受样品颜色、浊度、杂散光及酶联板自身光吸收不均一的影响；应用于测定三批共１１８份血清样品，阳性率分别为１５％（６／４０），６２．５％（２５／４０）和３１．５８％（１２／３８），用４６份样品对本法和ＥＬＩＳＡ作相关性检验，其相关性良好（γ＝０．７７．ｔ＿ｒ＝１０．８４，Ｐ＜０．００１，相关显著）。An electrochemical enzyme immunoassay for measles virus(MV) was based on heterogeneous enzyme immunoassay with polarographic detection. The measurement of the enzyme-catalyzed product is carried out directly in commercial microporous plate by linear-sweep polarography with a small three electrode system 'DME-Pt-Ag/AgCl'.The positive ratio determined by the proposed method in 118 sera specimens is higher than that by ELISA,and the good relativity of the proposed method wite RLISA(γ=0.77, tr=10.84,P<0.001)was observed by measuring 46 specimens.The proposed method bas high specificity, sensitivity, wide detection range,without the interferences of turbidity colour of samples,stray light and adsorption inhomogenity of micro-porous plate.

关 键 词：麻疹病毒 极谱 ELISA 抗体 

分 类 号：R511.1[医药卫生—内科学]