测定植物组织中金属硫肽含量的线性扫描极谱法  

Determination of Metallothio-peptide in Plant Tissues by Linear Sweep Polarography

作　　者：熊燚[1] 铁锋 丁翔 姜剑 李令媛[1] 茹炳根[1] 王英彦[2] 

机构地区：[1]北京大学生物系蛋白质工程国家实验室,北京100871 [2]北京市环境保护科学研究所,北京100037

出　　处：《分析化学》1994年第4期328-331,共4页Chinese Journal of Analytical Chemistry

基　　金：国家高技术863项目;北京市自然科学基金资助课题

摘　　要：本文介绍了线性扫描极谱法测定植物组织中金属硫肽含量的技术,其最小检出量为0.16μg/ml;线性范围为0.2～2.0μg/ml;回收率为90％左右。本文还对pH影响及巯基物质的干扰进行了分析。所有结果表明,本方法具有简易、快速、灵敏等特点。野外调测进一步表明,本方法适用于环境监测等实用分析。A rapid and easy technique of linear sweep polarography (LSP) is reported for the determination of metallothio-peptide in plant tissues. The detection limit is 0.16g/ml, the linear range of the concentration is from 0.2 to 2.0μg/ml and the recovery is between 87.1% and 97. 4%. Also, the effects of pH and thiol substances have been studied. The ex-perimental results on plants growing in natural water further show that th?LSP is practica-ble to environmental detection.

关 键 词：金属硫肽 线性扫描极谱 植物 含量 

分 类 号：Q944.62[生物学—植物学]