三波长分光光度法研究——Ⅱ.最佳测量波长组合的选择及两系数的确定  

Study on Three-wavelength Spectrophotometry Ⅱ. Selection of the Best Wavelength Combination for the Measurement and the Definition of Two Factors

作　　者：曹伟[1] 冯永健[1] 邹时复[2] 

机构地区：[1]山东建材学院应用化学系,济南250022 [2]山东大学化学系

出　　处：《分析化学》1994年第8期781-784,共4页Chinese Journal of Analytical Chemistry

摘　　要：本文讨论了三波长分光光度法测定混合三组份时波长组合的选择方法,以相对误差为标准,利用计算机在任意波长下选择并输出最佳测量波长组合,使三波长分光光度法的应用更加灵活方便.试验了两个三组份混合体系,均可获得较好的准确度与精密度。The selection of the wavelength combination of three-wavelength spectropho-tometry in the determination of three-component mixture is discussed. At arbitrary three wavelengths, the computer makes the difference of absorbance of two interential components zero, and evaluates K2 and K3,and then ΔA = Aλ1+K2Aλ2+K3Aλ3 is only linear with the concentration of the determined component. All wavelength combination in the determination range, and the relative error as standard are calculated, and the best wavelength combination is selected by computer. Two three-component mixtures of tryptophan,tyrosine,phenylala-nine and p-nitrophenol,o-nitrophenol, 2,4-dinitrophenol are determined with satisfactory results.

关 键 词：光度法 波长组合 多组分测定 

分 类 号：O657.3[理学—分析化学]